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  1. Feb 21, 2023 · Cultured primary cells and continuous cell lines are indispensable in investigations of basic, biomedical, and translation research. However, despite their important role, cell lines are frequently misidentified or contaminated by other cells, bacteria, fungi, yeast, viruses, or chemicals.

    • 10.3390/cells12050682
    • 2023/03
    • Cells. 2023 Mar; 12(5): 682.
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  3. Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use.

    Cell Line
    Meaning
    Organism
    Origin Tissue
    "3-day transfer, inoculum 3 x 10^5 ...
    Mouse
    Embryo
    Armenian Hamster Lung-1
    Hamster
    Lung
    BEAS-2B
    Bronchial epithelium + Adenovirus 12-SV40 ...
    Human
    Lung
    Brain Endothelial 3
    Mouse
    Brain/ cerebral cortex
    • 5%
    • 37 °C
    • 95%
  4. Apr 7, 2017 · Cell culture is one of the most important techniques in cellular and molecular biology since it provides a platform to investigate the biology, biochemistry, physiology (e.g., aging) and metabolism of wild-type cells and diseased cells.

    • Charis-P. Segeritz, Ludovic Vallier, Ludovic Vallier
    • 10.1016/B978-0-12-803077-6.00009-6
    • 2017
    • 2017
    • Cell Culture Definition
    • Cell Culture Applications
    • Cell Culture Protocol
    • Cell Culture Contamination
    • Quiz

    Cell culture is a method used to cultivate, propagate and grow a large amount of cells in a dish. The cells can be of a mixed, heterogeneous origin with different cell types growing, or they can be a singular cell type, sometimes clonal in origin. Cell culture allows one to grow cells outside of their natural environment and control the conditions ...

    Cell culture is widely used in basic and clinical research. It serves as a tool for cellular and molecular biology research where researchers can test drugs and growth conditions, and perform genetic manipulations on the cultured cell populations. Cell culture can be used to create a scaled-up source of a limiting population of cells found in-vivo,...

    Materials needed for cell culture: All steps of the protocol should be attempted in a cell culture hood that is maintained for aseptic technique to prevent contamination.

    Cell culture contamination happens when the cells become infected with bacteria, mycoplasma, yeast and/or mold. Using aseptic technique is important in preventing this type of contamination. It involves using a sterile work environment as much as possible, such as a designated cell culture fume hood that is disinfected after each use. The hood is o...

    1. Which of the following could be an application of cell culture? A. Large-scale cell production B. In-vivo genetic studies C. To study physical organ characteristics D.Drug production 2. Which of the following is a biological contaminant of cell culture? A. Endotoxins B. Mycoplasma C. Antibiotics D.Detergent 3. How do you know when a cell culture...

  5. Cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds on the cells, and mutagenesis and carcinogenesis.

  6. Mammalian cell culture is at the core of biomanufacturing therapeutic proteins and viral vaccines. Find out how mammalian cells are derived and cultivated, and what opportunities this field holds.

  7. Cell culture, the maintenance and growth of the cells of multicellular organisms outside the body in specially designed containers and under precise conditions of temperature, humidity, nutrition, and freedom from contamination.

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