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Loading... Feb 24, 2022 · Cell line. (Science: cell culture) a cell line is a permanently established cell culture that will proliferate indefinitely given appropriate fresh medium and space. lines differ from cell strains in that they have escaped the Hayflick limit and become immortalised.
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- Top 5 Commonly Used Cell Lines
- More Info on Commonly Used Cell Lines
- References
Number 5: Sf9 Cells
Well, we didn’t say that it would just be human cell lines, did we? Derived from the ovaries of the fall armyworm moth (Spodoptera frugiperda), these cells are probably related to all insect cell lines in labs worldwide. Sf9 insect epithelial cells can be cultured as adherent or suspension cells. Most cell lines are adherent cells, which grow only on the surfaces of culture vessels. This limits the number of cells you can expect to obtain from each culture. Similarly to E. coli, suspension ce...
Number 4: CHO cells
CHO, or Chinese hamster ovary cells, are clearly ovary-derived cells, but this time, we are talking about mammalian cells. Similarly to Sf9 cells, they can exist both as adherent or suspension cells in culture. CHO cells are used in many biological, medical, and pharmaceutical research applications, including recombinant protein production , and studies of the epidermal growth factor receptor.
Number 3: Insert Your Favorite Immortalized Human Cell Line Here!
Some may consider it cheating to use a cell line collection instead of a single cell line. But depending on your research area, you may use a collection such as those from the NCI-60 Human Tumor Cell Lines Screen. Alternatively, you might be interested in working with Jurkat or HL-60 (white blood cells), MCF-7 (breast cancer), Saos-2 cells (bone cancer), PC3 (prostate cancer), HepG2 (Liver cancer), or many others. Let’s move on to the top unquestionable leaders of our cell lines hit parade.
Not seen your favorite cell line here? Want to know how to get hold of a cell line or even generate one? Find out more in these related articles: 1. Microglial Cell Lines: The Ultimate Guide to Selection 2. The Culture of Primary Schwann Cells 3. Where To Get Your Perfect Cell Line 4. How to Become Immortal: Generation of Immortal Cell Lines Origin...
Vaughn JL et al. (1977). The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro 13(4):213–17.Cox MM (2012). Recombinant protein vaccines produced in insect cells. Vaccine 27; 30(10):1759–66.Wurm FM (2004). Production of recombinant protein therapeutics in cultivated mammalian cells. Nature Biotechnology 22:1393–8.Ahsan A et al. (2009). Role of Cell Cycle in Epidermal Growth Factor Receptor Inhibitor-Mediated Radiosensitization.Cancer Research 69(12):5108–14.The term cell line refers to the propagation of culture after the first subculture. In other words, once the primary culture is sub-cultured, it becomes a cell line. A given cell line contains several cell lineages of either similar or distinct phenotypes. ADVERTISEMENTS:
A cell line can be considered to be a cross between a “normal” cell and a cancer cell, both figuratively and sometimes literally. Cell lines are created in the laboratory to display the key characteristics of a specific cell type while at the same time being immortal.
Mar 9, 2023 · A cell line is a cell that has undergone mutation and series of genetic manipulations, and will not undergo apoptosis after a limited number of passages (sub-culturing). Apoptosis is defined as programmed cell death.
Cell lines are essential resources for biomedical research, clinical diagnostics, and clinical translation. A cell line is typically established from a tissue sample, placed into culture because an ongoing supply of cells is considered desirable.
Jan 1, 2012 · Cell lines are often used in place of primary cells to study biological processes. However, care must be taken when interpreting the results as cell lines do not always accurately replicate the primary cells. In this article, we will briefly talk about advantages and disadvantages of cell lines and then discuss results using the mouse Sertoli ...
