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Entamoeba coli, E. hartmanni, E. polecki, Endolimax nana, and Iodamoeba buetschlii are generally considered nonpathogenic and reside in the lumen of the large intestine in the human host. Both cysts and trophozoites of these species are passed in stool and are considered diagnostic .
E. coli trophozoites can be distinguished by their wide and tapered pseudopodia. They are often mistaken for E. histolytica due to their overlap in size. The cysts are distinguished by noticing the eight nuclei found in the mature form. To diagnose for E. coli, a stool sample is usually tested.
Cysts and trophozoites are passed in feces . Cysts are typically found in formed stool, whereas trophozoites are typically found in diarrheal stool. Infection with Entamoeba histolytica (and E.dispar) occurs via ingestion of mature cysts from fecally contaminated food, water, or hands.
May 3, 2016 · Table 1: Characteristics of Intestinal Amebae Visible in Different Types of Fecal Preparations. Quensel’s stain may be substituted for buffered methylene blue. Nuclei of trophozoites are visible in formalin-fixed material but are usually not sufficiently distinctive for species identification.
The trophozoite is larger than that of E. histolytica ranging from 15-50 in diameter. It exhibits blunt pseudopodia with sluggish movement. A permanently stained preparation shows a nucleus with a moderately large eccentric karyosome with the chromatin clumped on the nuclear membrane.
Entamoeba coli has three distinct morphological forms: trophozoite, pre-cystic stage, and cystic stage. The most distinguishing feature is the eight-core nuclei, which develops as the cyst begins to mature.
Jan 1, 2018 · Entamoeba coli is a non-pathogenic amoeba (protozoa) with world wide distribution; life cycle: similar to that of E. histolytica but it does not have an invasive stage and does not ingest red blood cells; organisms exists as a motile trophozoite and a cyst
Feb 13, 2017 · Entamoeba histolytica occurs in the following forms, the trophozoites, precyst, cyst, metacyst, and metacystic trophozoite. The trophozoite is about 10–60 μm in size with a clear finely granular cytoplasm and a spherical nucleus that shows aggregation of chromatin beads at the nuclear membrane and a centrally located karyosome.
Trophozoites of Entamoeba coli usually measure 15 to 50 µm. The trophozoites possess a single nucleus with a characteristically large, eccentric karyosome and coarse, irregular peripheral chromatin. The cytoplasm is usually coarsely granular and vacuolated (often described as "dirty" cytoplasm).
Parasitic amoebae of the genus Entamoeba are transmitted from one host to another as cysts. The active amoeboid forms are so adapted to life within their particular hosts that, outside the host, they soon die, but encysted stages survive conditions fatal to the unprotected amoebae.
