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Feb 28, 2022 · Dead volume actually refers to “volumes within the chromatographic system which are not swept by the mobile phase.” 1. Extra column volume – all volume with an HPLC system from the sample loop to the detector, excluding the column. Dead volume contributes a portion of this volume. Dwell volume – a.k.a gradient delay volume, is the ...
Sep 13, 2023 · 1. Broadens peak s. Dead volume can broaden peaks, lower peak resolution and decrease the separation between your closely eluting compounds. This change makes quantifying the analytes difficult and thus reduces your confidence in the data set. 2. Reduces sensitivity. Broader peaks can lead to decreased sensitivity.
Dead volume is the volume of solution left in the needle after an injection. Learn how to calculate dead volume and prime it with sample for precise dispensing with Hamilton syringes.
Nov 1, 2015 · Dead volume is the volume of mobile phase that solutes experience outside of the column in chromatography. Learn how dead volume can affect peak shape, resolution, and quantification in GC and LC, and how to minimize it.
Jun 2, 2023 · Dead volume, or the un-agitated fluid in bioreactors, can affect cell culture processes and product yield, thereby influencing the effectiveness and profitability of biotechnological operations. Dead Volume: A Key Player in Chromatographic Separation Efficiency. In the world of chromatography, dead volume holds a critical position. Its role is ...
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What is dead volume in HPLC?
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What is dead volume?
What is the difference between dead volume and extra column volume?
Jan 23, 2024 · 2. Techniques for Adjusting Dead Volume to Navigate Peak Constraints: Peak broadening: In rare cases where peak broadening is beneficial (e.g., trace-level analysis), slightly increase dead volume by using wider-bore tubing or larger tubing lengths. However, carefully balance this with the potential loss of resolution.
Apr 30, 2019 · Extra dead-volume can be the thing of nightmares for your chromatography if not properly minimized in your LC system. So, let us face our fears together and defeat dead-volume with Phenomenex new finger tight connection solution— SecurityLINKs! Often when using the standard HPLC column dimensions of 150 x 4.6 mm with a 5 µm particle you don ...
