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4 days ago · Bacteriophage infection, a pivotal process in microbiology, initiates with the phage’s tail recognizing and binding to the bacterial cell surface, which then mediates the injection of viral DNA.
4 days ago · The hepatic abundance of cytochrome P450 (CYP) enzymes reflects their major contribution to metabolic biotransformation. This contribution is not limited to xenobiotics, e.g. drugs, metabolism , but it also extends to catalyzing homeostatic transformation of endobiotics . Additionally, the abundance of hepatic CYP monooxygenases makes them more ...
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5 days ago · Biosynthesis of paclitaxel (Taxol™) is a hot topic with extensive and durable interests for decades. However, it is severely hindered due to the very low titers of intermediates. In this study, Escherichia coli was employed to de novo synthesize a key intermediate of paclitaxel, taxadien-5α-yl-acetate (T5OAc). Plasmid-based pathway reconstruction and optimization were conducted for T5OAc ...
2 days ago · Betalain biosynthesis requires three key enzymes: P450 oxygenase CYP76AD1, l-3,4-dihydroxyphenylalanine (l-DOPA) 4,5-dioxygenase (DODA), and glycosyltransferase (GT). In the RUBY system, a single mRNA encoding these three enzymes linked by 2A peptides is transcribed, and then the three enzymes are translated into proteins that catalyze betalain ...
5 days ago · The animal room had controlled temperature (18-23°C) and humidity (40-60%) and a 12 h light/12 h dark cycle. METHOD DETAILS Plasmid construction and protein purification His-tagged full length SIRT7 protein expression from BL21 bacterial cells was induced by the addition of 0.25 mM isopropyl β-D-1-thiogalactopyranoside (IPTG).
1 day ago · Anatomy and infection cycle of bacteriophage T4. A bacteriophage ( / bækˈtɪərioʊfeɪdʒ / ), also known informally as a phage ( / ˈfeɪdʒ / ), is a virus that infects and replicates within bacteria and archaea. The term was derived from "bacteria" and the Greek φαγεῖν ( phagein ), meaning "to devour".
5 days ago · Bacterial genetics is the study of the mechanisms of heritable information in bacteria, their chromosomes, plasmids, transposons and phages. Techniques that have enabled this discipline are ...
