Isabella Clara Eugenia of Austria was born in the Palacio del bosque de Valsaín, Segovia on 12 August 1566. She was the first surviving daughter of King Philip II of Spain and his third wife, Elisabeth of Valois.
Christina of Lorraine or Christine de Lorraine (16 August 1565 – 19 December 1637) was a member of the House of Lorraine and was the Grand Duchess of Tuscany by marriage. . She served as Regent of Tuscany jointly with her daughter-in-law during the minority of her grandson from 1621 to 1