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Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR).
PCR is a simple, yet elegant, enzymatic assay, which allows for the amplification of a specific DNA fragment from a complex pool of DNA. Dr. Kary Mullis, who discovered the PCR assay, stated it “lets you pick the piece of DNA you’re interested in and have as much of it as you want” ( Mullis, 1990 ).
Apr 22, 1999 · Kary Mullis (born December 28, 1944, Lenoir, North Carolina, U.S.—died August 7, 2019, Newport Beach, California) was an American biochemist, cowinner of the 1993 Nobel Prize for Chemistry for his invention of the polymerase chain reaction (PCR), a simple technique that allows a specific stretch of DNA to be copied billions of times in a few hours.
The versatility of polymerase chain reaction (PCR) has led to modifications of the basic protocol being used in a large number of variant techniques designed for various purposes.
Jul 14, 2021 · Contents. 1 All PCR. 2 end-point PCR. 3 Real-time PCR. 4 Link. All PCR. A thermal cycler for PCR. Schematic drawing of the PCR cycle. Not entirely accurate (see description). Diagram of first four PCR cycles. Electrophoresis of PCR-amplified DNA fragments. Cloning a gene using a plasmid.
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Kary Banks Mullis (December 28, 1944 – August 7, 2019) was an American biochemist.In recognition of his role in the invention of the polymerase chain reaction (PCR) technique, he shared the 1993 Nobel Prize in Chemistry with Michael Smith and was awarded the Japan Prize in the same year. PCR became a central technique in biochemistry and molecular biology, described by The New York Times as ...
