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The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract.
- Nitrocellulose
Nitrocellulose film on a light box, showing deterioration,...
- Northern Blot
The northern blot, or RNA blot, is a technique used in...
- Southern Blot
Agarose gel Tray with a stack consisting top down of a...
- Nitrocellulose
Sep 12, 2021 · Further, with Western blot, we found that consistent with microarray pathway assay, Antagomir425 inhibited PI3K‐Akt signaling and its effects could be reversed by PI3K‐Akt pathway activator SC79. Meanwhile, Agomir425 promoted Akt phosphorylation and its effects could be inhibited by PI3K‐Akt pathway inhibitor LY294002 (Figure S5). The ...
- Yong‐Bo Hu, Yong‐Bo Hu, Yong‐Fang Zhang, Ru‐Jing Ren, Eric B. Dammer, Xin‐Yi Xie, Shi‐Wu Chen, Qiang...
- 10.1111/acel.13454
- 2021
- Aging Cell. 2021 Oct; 20(10): e13454.
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Nov 7, 2019 · 1. Introduction. The western blot is a common method used to detect proteins as well as post-translational modifications on proteins, and can provide semi-quantitative or quantitative data about the target protein in simple or complex biological samples [ ]. Western blotting is an extensively used technique for protein analysis [ – ].
- Manish Mishra, Shuchita Tiwari, Aldrin V. Gomes
- 10.1080/14789450.2017.1388167
- 2017
- 2017/11
By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells. The technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target protein using a proper primary and secondary antibody to visualize.
- Tahrin Mahmood, Ping-Chang Yang
- 10.4103/1947-2714.100998
- 2012
- N Am J Med Sci. 2012 Sep; 4(9): 429-434.
Western blot, or western blotting, is a technique widely used in research to separate and identify specific proteins within a complex mixture. Western blot allows us to determine the relative protein levels between samples and establish the molecular weight of the target, which can provide insight into its post-translational processing.
Western blotting is a technique that uses specific antibodies to identify proteins separated by size through gel electrophoresis. The immunoassay uses a membrane made of nitrocellulose or PVDF (polyvinylidene fluoride). The gel is placed next to the membrane, and an electrical current is applied.
Western blot, or western blotting, is a technique widely used in research to separate and identify specific proteins within a complex mixture. Western blot allows us to determine the relative protein levels between samples and establish the molecular weight of the target, which can provide insight into its post-translational processing.
