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Sep 12, 2021 · Results confirmed a higher methylation of the miR‐425 gene promoter in the hippocampus of AD patients, with reduced miR‐425 expression, strengthening a hypothesis that miR‐425 may suppress APAM required for AD pathology (Figure 2k,l).
- Yong‐Bo Hu, Yong‐Bo Hu, Yong‐Fang Zhang, Ru‐Jing Ren, Eric B. Dammer, Xin‐Yi Xie, Shi‐Wu Chen, Qiang...
- 10.1111/acel.13454
- 2021
- Aging Cell. 2021 Oct; 20(10): e13454.
Western blot. The western blot (sometimes called the protein immunoblot ), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. [1]
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Nov 7, 2019 · Go to: 1. Introduction. The western blot is a common method used to detect proteins as well as post-translational modifications on proteins, and can provide semi-quantitative or quantitative data about the target protein in simple or complex biological samples [ 1 ].
- Manish Mishra, Shuchita Tiwari, Aldrin V. Gomes
- 10.1080/14789450.2017.1388167
- 2017
- 2017/11
Western blot is a technique that is very useful for protein detection as it allows the user to quantify the protein expression as well. This paper covered the protocol, the theory behind that protocol, and some troubleshooting techniques.
- Tahrin Mahmood, Ping-Chang Yang
- 10.4103/1947-2714.100998
- 2012
- N Am J Med Sci. 2012 Sep; 4(9): 429-434.
Introduction to western blot. Find out the difference between indirect and direct labeling in western blot and learn different methods of immunoblot detection.
What is a western blot? The term "blotting" refers to the transfer of biological samples from a gel to a membrane and their subsequent detection on the surface of the membrane.
Sep 18, 2022 · Western blotting, which originated from the laboratory of George Stark at Stanford, is the technique for detecting a particular protein by staining with specific antibody. Its analysis can give us the information about the size of the protein or how much protein accumulated in cells.