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The western blot (sometimes called the protein immunoblot ), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. [1] Besides detecting the proteins, this technique is also utilized to visualize, distinguish, and quantify the ...
- Nitrocellulose
Nitrocellulose film on a light box, showing deterioration,...
- Northern Blot
The northern blot, or RNA blot, is a technique used in...
- Southern Blot
Agarose gel Tray with a stack consisting top down of a...
- Nitrocellulose
Sep 12, 2021 · (e) Western blot of APP, BACE1, sAPPα/β, CTF‐α, and CTF‐β in the brain of miR‐425 +/− and WT mice. (f) ELISA of Aβ40 and CTF‐β in the brain of 15‐month‐old Mir425 +/− and WT mice. (g, h) Western blot of APP and CTF‐β in PC12 cells transfected with Antagomir‐425 and Agomir‐425.
- Yong‐Bo Hu, Yong‐Bo Hu, Yong‐Fang Zhang, Ru‐Jing Ren, Eric B. Dammer, Xin‐Yi Xie, Shi‐Wu Chen, Qiang...
- 10.1111/acel.13454
- 2021
- Aging Cell. 2021 Oct; 20(10): e13454.
Western blotting is an important technique used in cell and molecular biology. By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells. The technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking ...
- Tahrin Mahmood, Ping-Chang Yang
- 10.4103/1947-2714.100998
- 2012
- N Am J Med Sci. 2012 Sep; 4(9): 429-434.
Western Blot (WB) is a common method to detect and analyze proteins. It is built on a technique that involves transferring, also known as blotting, proteins separated by electrophoresis from the gel to a membrane where they can be visualized specifically. The procedure was first described by H. Towbin et al in 1979 ( Towbin, Staehelin, & Gordon ...
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Nov 7, 2019 · 1. Introduction. The western blot is a common method used to detect proteins as well as post-translational modifications on proteins, and can provide semi-quantitative or quantitative data about the target protein in simple or complex biological samples [ ]. Western blotting is an extensively used technique for protein analysis [ – ].
- Manish Mishra, Shuchita Tiwari, Aldrin V. Gomes
- 10.1080/14789450.2017.1388167
- 2017
- 2017/11
Western blotting identifies with specific antibodies proteins that have been separated from one another according to their size by gel electrophoresis. The blot is a membrane, almost always of nitrocellulose or PVDF (polyvinylidene fluoride). The gel is placed next to the membrane and application of an electrical current induces the proteins in ...
Western blot, or western blotting, is a technique widely used in research to separate and identify specific proteins within a complex mixture. Western blot allows us to determine the relative protein levels between samples and establish the molecular weight of the target, which can provide insight into its post-translational processing.
