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4 days ago · Western Blot Analysis is the most widely used technique for protein detection. In this video, we will cover the 3 major steps in generating a western blot: Separate, Transfer, and Detect.
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Apr 17, 2024 · Protein modifications, low signal, poor consistency, and high background can lead to suboptimal Western blotting results. We will outline a comprehensive approach to experimental design and explore improvements in technology that have made blotting more user-friendly and efficient.
5 days ago · In this video, you’ll learn how to perform a western transfer using the Mini Blot module in a Mini Gel Tank. Make sure the cassette clamps are removed before you set up your transfers. One gel is transferred per module, and two modules fit in one tank.
- 4 min
Apr 28, 2024 · Introduction: Western blotting is a technique used to identify and locate proteins based on their ability to bind to specific antibodies. Western blot analysis can detect your protein of interest from a mixture of a number of proteins.
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Apr 15, 2024 · Western Blot Detection. You’ve probably been there. You’ve got a new antibody or you’re testing out one you’ve made yourself. After weeks or months of work, your antibody is going to help move your research project forward. As you excitedly head to the dark room to develop your film, your mood is crushed when you see…bands, more bands, and smears.
