Search results
Below are videos that detail the common steps used to perform a western blot, from separating proteins in protein gel electrophoresis to developing the blot. Step 1 Gel electrophoresis. Step 2 Membrane transfer. Step 3 Probing. Step 4 Detection. Step 5 Imaging and analysis. Step 6 Stripping. Separate proteins based on molecular weight. / Loaded 0%
Western Blot video protocol by Proteintech Group. Western blot is a widely used analytical technique used to detect specific proteins in the given sample of tissue homogenate or extract. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide (denaturing conditions) or by the 3-D structure of the ...
Mar 28, 2023 · Running a Western blot can be a bit daunting for first-timers. That's why we gave our written protocol a video companion! Follow along as Addgenie Meghan and Abi run a Western blot and share tips for this tricky application. You can check out all of our protocols on our Protocol page, and find our protocol videos on our YouTube page . Topics ...
Protein transfer and visualization in western blot. Detailed protocol for the transfer and staining of proteins in western blot. Includes visualization of proteins in gels, transfer, and development methods. Fluorescent western blot. Get started with fluorescent western blot with these quick tips. Membrane stripping for western blot. Essential ...
Western Blot Video. (These may take a moment to load. It will be worth the wait!) In this visual protocol series, learn how learn how to perform all phases of a western blot using the most common methods for this assay. Phase One - Sample Preparation. Learn how to prepare a sample lysate from cell culture.
Language links are at the top of the page across from the title.
View the Video. Short videos from CST highlighting our optimized western blotting protocol and troubleshooting guide to diagnose issues and recommend solutions.
