Search results
The western blot (sometimes called the protein immunoblot ), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. [1] Besides detecting the proteins, this technique is also utilized to visualize, distinguish, and quantify the ...
- Nitrocellulose
Nitrocellulose film on a light box, showing deterioration,...
- Northern Blot
The northern blot, or RNA blot, is a technique used in...
- Southern Blot
Agarose gel Tray with a stack consisting top down of a...
- Nitrocellulose
Sep 12, 2021 · (e) Western blot of APP, BACE1, sAPPα/β, CTF‐α, and CTF‐β in the brain of miR‐425 +/− and WT mice. (f) ELISA of Aβ40 and CTF‐β in the brain of 15‐month‐old Mir425 +/− and WT mice. (g, h) Western blot of APP and CTF‐β in PC12 cells transfected with Antagomir‐425 and Agomir‐425.
- Yong‐Bo Hu, Yong‐Bo Hu, Yong‐Fang Zhang, Ru‐Jing Ren, Eric B. Dammer, Xin‐Yi Xie, Shi‐Wu Chen, Qiang...
- 10.1111/acel.13454
- 2021
- Aging Cell. 2021 Oct; 20(10): e13454.
People also ask
What is a western blot?
What is a result analysis in a western blot experiment?
What is a western blotting assay?
Should total protein staining be used in western blot analysis?
Feb 8, 2023 · The Stages of Western Blotting. The western blotting assay is mainly composed of seven main steps: Sample Preparation: Cell or tissue lysate of your choice of the cell line, or any other protein sample of choice is prepared to isolate and analyze specific proteins and loaded on SDS-PAGE. Electrophoretic separation of Proteins: The total protein ...
Nov 7, 2019 · 1. Introduction. The western blot is a common method used to detect proteins as well as post-translational modifications on proteins, and can provide semi-quantitative or quantitative data about the target protein in simple or complex biological samples [ ]. Western blotting is an extensively used technique for protein analysis [ – ].
- Manish Mishra, Shuchita Tiwari, Aldrin V. Gomes
- 10.1080/14789450.2017.1388167
- 2017
- 2017/11
Apr 14, 2023 · The name ‘western’ blot was first coined by Dr. Burnette in 1981 after the eponymous Southern blot for DNA and the consequent coinage of the northern blot in 1977 for RNA.[1][2] Western blotting separates, detects, and identifies one or more proteins in a complex mixture.[3] It involves separating the individual proteins by polyacrylamide gel electrophoresis and then transferring or ...
Protein transfer and visualization in western blot. Detailed protocol for the transfer and staining of proteins in western blot. Includes visualization of proteins in gels, transfer, and development methods. Fluorescent western blot. Get started with fluorescent western blot with these quick tips. Membrane stripping for western blot. Essential ...
Key steps for achieving sensitive chemiluminescent quantitative western blots. Traditional western blotting is useful for detecting the presence or absence of a specific protein in a lysate or complex mixture. Furthermore, it can provide a qualitative assessment of changes in protein expression levels in response to stimuli or interventions.
