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  1. The genome sequence of E. coli predicts 4288 protein-coding genes, of which 38 percent initially had no attributed function. Comparison with five other sequenced microbes reveals ubiquitous as well as narrowly distributed gene families; many families of similar genes within E. coli are also evident.

    • EPEC

      Escherichia coli (/ ˌ ɛ ʃ ə ˈ r ɪ k i ə ˈ k oʊ l aɪ /...

    • Entamoeba COLI

      Entamoeba coli is a non-pathogenic species of Entamoeba that...

    • Gram-negative

      Microscopic image of gram-negative Pseudomonas aeruginosa...

    • Enterobacteriaceae

      Escherichia coli is one of the most important model...

  2. Escherichia coli (commonly abbreviated E. coli) is a Gram-negative, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some serotypes can cause serious food poisoning in humans.

  3. Morphology and Staining of Escherichia Coli: E. coli is Gram-negative straight rod, 1-3 µ x 0.4-0.7 µ, arranged singly or in pairs (Fig. 28.1). It is motile by peritrichous flagellae, though some strains are non-motile.

    • Diseases Caused by E. Coli
    • Characteristics of E. Coli
    • Virulence Factors of E.coli
    • Diagnostic Features of E.coli
    • Antimicrobial Susceptibility Testing
    Urinary tract infections (UTI): E.coli is the most common cause of both community and nosocomial urinary tract infections; E.coli causes more than 75% of cases of UTI.
    E.coli is the second most important cause of Gram-negative rod sepsis
    Perinatal infection with E.coli (exposure of newborn to E.coli colonized in the birth canal of the mother during natural birth) is the predominant cause of neonatal meningitis
    Enterotoxigenic Escherichia coli (ETEC) is responsible for the traveler’s diarrhea(watery diarrhea)
    Gram stain: Escherichia coliis a straight gram-negative short rod or bacilli
    Escherichia coli cells are small rods 1.0-2.0 micrometers long, with a radius of about 0.5 micrometers. However, the size varies with the medium, and faster-growing cells are larger.
    E. coliis the most abundant facultative anaerobe in the colon and feces.
    The generation (doubling) time of Escherichia coliis 20 minutes.
    Pili:Helps in adherence of organisms to the cells of jejunum and ileum in case of intestinal tract infection; urinary tract epithelium in case of urinary tract infections.
    Endotoxin (lipopolysaccharide): Responsible for several features of gram-negative sepsis such as fever, hypotension, and disseminated intravascular coagulation (DIC).
    Exotoxinse.g. enterotoxin act on the cells of the jejunum and ileum to cause diarrhea. Other exotoxins are verotoxin, Shiga-like toxin, etc.
    Colony Morphology: E.coli ferments lactose and produces pink colonies on MacConkey Agar. Typical colonies of Escherichia coli on MacConkey agar will appear pink and shiny and have a diameter of 0.5...
    On EMB agar, E. coliproduces a characteristic green sheen.

    Perform the susceptibility test by a disc-diffusion method using standard methods described in the guidelines. To detect ESBL-producing E.coli, the isolate screened should be multidrug-resistant exhibiting resistance to at least one of the third-generation cephalosporins. Extended-spectrum ß-lactamases (ESBL) are a rapidly evolving group of ß-lacta...

  4. Jan 1, 2008 · E. coli is Gram-negative and its envelope has three layers: cytoplasmic membrane, peptidoglycan, and outer membrane. The peptidoglycan is rigid determining the rod shape. To a good approximation, the E. coli cell has hemispherical caps and a cylindrical section in between.

    • Galina Reshes, Sharon Vanounou, Itzhak Fishov, Mario Feingold
    • 10.1529/biophysj.107.104398
    • 2008
    • Biophys J. 2008 Jan 1; 94(1): 251-264.
  5. Dec 31, 2022 · Most notably, E. coli are lactose, catalase, and indole positive, and oxidase, urease, and citrate negative, although there is a low level of polymorphism for many of these properties.

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  7. Mar 7, 2017 · We report 111 nonessential mutations impacting E. coli morphology, with nearly half of those genes being poorly annotated or uncharacterized. Further, these genes appear to be tightly linked to transport or redox processes within the cell.

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