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  1. Escherichia coli ( / ˌɛʃəˈrɪkiə ˈkoʊlaɪ / ESH-ə-RIK-ee-ə KOH-ly) [1] [2] is a gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms. [3] [4] Most E. coli strains are harmless, but some serotypes such as EPEC, and ETEC are ...

    • EPEC

      Escherichia coli (/ ˌ ɛ ʃ ə ˈ r ɪ k i ə ˈ k oʊ l aɪ /...

    • Entamoeba COLI

      Entamoeba coli is a non-pathogenic species of Entamoeba that...

    • Gram-negative

      Microscopic image of gram-negative Pseudomonas aeruginosa...

    • Diseases Caused by E. Coli
    • Characteristics of E. Coli
    • Virulence Factors of E.coli
    • Diagnostic Features of E.coli
    • Antimicrobial Susceptibility Testing
    E.coli is the second most important cause of Gram-negative rod sepsis
    Perinatal infection with E.coli (exposure of newborn to E.coli colonized in the birth canal of the mother during natural birth) is the predominant cause of neonatal meningitis
    Enterotoxigenic Escherichia coli (ETEC) is responsible for the traveler’s diarrhea(watery diarrhea)
    Gram stain: Escherichia coliis a straight gram-negative short rod or bacilli
    Escherichia coli cells are small rods 1.0-2.0 micrometers long, with a radius of about 0.5 micrometers. However, the size varies with the medium, and faster-growing cells are larger.
    E. coliis the most abundant facultative anaerobe in the colon and feces.
    The generation (doubling) time of Escherichia coliis 20 minutes.
    Pili:Helps in adherence of organisms to the cells of jejunum and ileum in case of intestinal tract infection; urinary tract epithelium in case of urinary tract infections.
    Endotoxin (lipopolysaccharide): Responsible for several features of gram-negative sepsis such as fever, hypotension, and disseminated intravascular coagulation (DIC).
    Exotoxinse.g. enterotoxin act on the cells of the jejunum and ileum to cause diarrhea. Other exotoxins are verotoxin, Shiga-like toxin, etc.
    Colony Morphology: E.coli ferments lactose and produces pink colonies on MacConkey Agar. Typical colonies of Escherichia coli on MacConkey agar will appear pink and shiny and have a diameter of 0.5...
    On EMB agar, E. coliproduces a characteristic green sheen.

    Perform the susceptibility test by a disc-diffusion method using standard methods described in the guidelines. To detect ESBL-producing E.coli, the isolate screened should be multidrug-resistant exhibiting resistance to at least one of the third-generation cephalosporins. Extended-spectrum ß-lactamases (ESBL) are a rapidly evolving group of ß-lacta...

  2. Jan 1, 2008 · Bacteria are the simplest living organisms. In particular, Escherichia coli has been extensively studied and it has become one of the standard model systems in microbiology. However, optical microscopy studies of single E. coli have been limited by its small size, ∼1 × 3 μm, not much larger than the optical resolution, ∼0.25 μm.

    • Galina Reshes, Sharon Vanounou, Itzhak Fishov, Mario Feingold
    • 10.1529/biophysj.107.104398
    • 2008
    • Biophys J. 2008 Jan 1; 94(1): 251-264.
  3. Dec 31, 2022 · Abstract. Escherichia coli have served as important model organisms for over a century—used to elucidate key aspects of genetics, evolution, molecular biology, and pathogenesis. However, defining which strains actually belong to this species is erratic and unstable due to shifts in the characters and criteria used to distinguish bacterial ...

  4. Jun 1, 2015 · The originality of this work lays on addressing colony morphotyping in a systematic, harmonised and computerised way. Although still in its infancy, MorphoCol aims to pave the way to the development of advanced clinical decision making applications, which may use morphological features as immediate indicators of microbial behaviour (Fig. 1).

    • Ana Margarida Sousa, Maria Olívia Pereira, Anália Lourenço, Anália Lourenço
    • 2015
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  6. Jan 7, 2024 · Colony morphology can sometimes be useful in bacterial identification. Colonies are described based on size, shape, texture, elevation, pigmentation, and effect on growth medium. Find common criteria that are used to characterize bacterial growth; Colony shape. Size of the bacterial colony. Appearance of the colony surface.

  7. Aug 6, 2013 · Escherichia coli can grow and divide in a wide range of pressure (1–400 atm) and temperature (23–40°C). For T > 30°C, the doubling time of E. coli increases exponentially with pressure and exhibits a departure from exponential behavior at pressures between 250 and 400 atm for all the temperatures studied in our experiments.

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