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Escherichia coli ( / ˌɛʃəˈrɪkiə ˈkoʊlaɪ / ESH-ə-RIK-ee-ə KOH-ly) [1] [2] is a gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms. [3] [4] Most E. coli strains are harmless, but some serotypes such as EPEC, and ETEC are ...
- EPEC
Escherichia coli (/ ˌ ɛ ʃ ə ˈ r ɪ k i ə ˈ k oʊ l aɪ /...
- Entamoeba COLI
Entamoeba coli is a non-pathogenic species of Entamoeba that...
- Gram-negative
Microscopic image of gram-negative Pseudomonas aeruginosa...
- Enterobacteriaceae
Enterobacteriaceae is a large family of Gram-negative...
- Salmonella Enterica
Salmonella enterica (formerly Salmonella choleraesuis) is a...
- Facultative Anaerobic
Functions. Facultative anaerobes are able to grow in both...
- EPEC
Endo agar. Endo agar (also called Endo's medium) is a microbiological growth medium with a faint pink colour. [1] Originally developed for the isolation of Salmonella typhi, it is now used mostly as a coliform medium. Most gram-negative organisms grow well in this medium, while growth of gram-positive organisms is inhibited. [2]
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May 17, 2018 · Shape – Escherichia coli is a straight, rod shape (bacillus) bacterium. Size – The size of Escherichia coli is about 1–3 µm × 0.4–0.7 µm (micrometer). Arrangement Of Cells – Escherichia coli is arranged singly or in pairs. Motility – Escherichia coli is a motile bacterium. Some strains of E. coli are non-motile.
Escherichia coli. Four different strains of Escherichia coli on Endo agar with biochemical slope . Glucose fermentation with gas production, urea and H 2 S negative, lactose positive (with exception of strain D - "late lactose fermenter"; on Endo agar it looks like lactose negative).
- Principle of Endo Agar
- Preparation and Method of Use of Endo Agar
- Uses of Endo Agar
- Limitations of Endo Agar
- References
The medium contains peptone which provides nitrogen, carbon, vitamins, and minerals required for bacterial growth.Sodium sulfite and basic fuchsin make this medium selective by suppressing gram-positive organisms.Lactose positive colonies exhibit a red color caused by the aldehyde reaction with the Sodium Sulfite and Basic Fuchsin.The development of a metallic sheen occurs when the organism produces aldehydes with the fermentation of Lactose. Lactose non-fermenting bacteria form clear, colorless colonies.Suspend 41.5 grams in 1000 ml distilled water.Heat to boiling to dissolve the medium completely.Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.Mix well before pouring into sterile Petri plates.The medium is now used for the differentiation of lactose fermenting and non-lactose fermenting intestinal organisms, particularly during confirmation of the presumptive test for coliforms.Endo Agar is recommended by APHA as an important medium in the microbiological examination of water and wastewater, dairy products and foods.Endo Agar is used to confirming the detection and enumeration of coliform bacteria following presumptive test of drinking water.It is also used for the detection and isolation of coliforms and fecal coliforms from milk, dairy products, and food.Besides Enterobacteriaceae, other gram-negative bacteria and yeasts may also grow.Avoid exposure of the medium to light, as it may lead to photooxidation and decrease the productivity of the medium.Overheating of the medium must be avoided, as it may destroy the productivity of the medium.Further biochemical tests must be carried out for further confirmation.Escherichia coli have an incubation period of 12–72 hours with the optimal growth temperature being 37 °C. Unlike the general coliform group, E. coli are almost exclusively of fecal origin and their presence is thus an effective confirmation of fecal contamination. Most strains of E. coli are harmless, but some can cause serious illness in ...
Dec 27, 2023 · Escherichia coli cells are small rods 1.0-2.0 micrometers long, with a radius of about 0.5 micrometers. However, the size varies with the medium, and faster-growing cells are larger. E. coli is the most abundant facultative anaerobe in the colon and feces. The generation (doubling) time of Escherichia coli is 20 minutes.
