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Type and morphology. E. coli is a gram-negative, facultative anaerobe, nonsporulating coliform bacterium. Cells are typically rod-shaped, and are about 2.0 μm long and 0.25–1.0 μm in diameter, with a cell volume of 0.6–0.7 μm 3.
- EPEC
Escherichia coli ( / ˌɛʃəˈrɪkiə ˈkoʊlaɪ / ESH-ə-RIK-ee-ə...
- Entamoeba COLI
Entamoeba coli is a non-pathogenic species of Entamoeba that...
- Gram-negative
Microscopic image of gram-negative Pseudomonas aeruginosa...
- Enterobacteriaceae
Enterobacteriaceae is a large family of Gram-negative...
- Salmonella Enterica
Salmonella enterica (formerly Salmonella choleraesuis) is a...
- Facultative Anaerobic
Functions. Facultative anaerobes are able to grow in both...
- EPEC
Apr 4, 2022 · This review highlights the recent discoveries in EEC differentiation and function while also discussing newly revealed roles of transcription factors and signaling networks involved in the allocation of EEC subtypes that were discovered using a combination of novel intestinal model systems and genetic sequencing.
- 10.1097/MED.0000000000000709
- 2022/04/04
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- Principle of Endo Agar
- Preparation and Method of Use of Endo Agar
- Uses of Endo Agar
- Limitations of Endo Agar
- References
The medium contains peptone which provides nitrogen, carbon, vitamins, and minerals required for bacterial growth.Sodium sulfite and basic fuchsin make this medium selective by suppressing gram-positive organisms.Lactose positive colonies exhibit a red color caused by the aldehyde reaction with the Sodium Sulfite and Basic Fuchsin.The development of a metallic sheen occurs when the organism produces aldehydes with the fermentation of Lactose. Lactose non-fermenting bacteria form clear, colorless colonies.Suspend 41.5 grams in 1000 ml distilled water.Heat to boiling to dissolve the medium completely.Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.Mix well before pouring into sterile Petri plates.The medium is now used for the differentiation of lactose fermenting and non-lactose fermenting intestinal organisms, particularly during confirmation of the presumptive test for coliforms.Endo Agar is recommended by APHA as an important medium in the microbiological examination of water and wastewater, dairy products and foods.Endo Agar is used to confirming the detection and enumeration of coliform bacteria following presumptive test of drinking water.It is also used for the detection and isolation of coliforms and fecal coliforms from milk, dairy products, and food.Besides Enterobacteriaceae, other gram-negative bacteria and yeasts may also grow.Avoid exposure of the medium to light, as it may lead to photooxidation and decrease the productivity of the medium.Overheating of the medium must be avoided, as it may destroy the productivity of the medium.Further biochemical tests must be carried out for further confirmation.Dec 31, 2022 · Escherichia coli have served as important model organisms for over a century—used to elucidate key aspects of genetics, evolution, molecular biology, and pathogenesis. However, defining which strains actually belong to this species is erratic and unstable due to shifts in the characters and criteria used to distinguish bacterial species.
- Marta Cobo-Simón, Rowan Hart, Howard Ochman
- Mol Biol Evol. 2023 Jan; 40(1): msac273.
- 10.1093/molbev/msac273
- 2023/01
Mar 7, 2017 · We report 111 nonessential mutations impacting E. coli morphology, with nearly half of those genes being poorly annotated or uncharacterized. Further, these genes appear to be tightly linked to transport or redox processes within the cell.
In microbiology, colonial morphology refers to the visual appearance of bacterial or fungal colonies on an agar plate. Examining colonial morphology is the first step in the identification of an unknown microbe.
