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  1. Type and morphology. E. coli is a gram-negative, facultative anaerobe, nonsporulating coliform bacterium. Cells are typically rod-shaped, and are about 2.0 μm long and 0.25–1.0 μm in diameter, with a cell volume of 0.6–0.7 μm 3.

    • EPEC

      Escherichia coli (/ ˌ ɛ ʃ ə ˈ r ɪ k i ə ˈ k oʊ l aɪ /...

    • Entamoeba COLI

      Entamoeba coli is a non-pathogenic species of Entamoeba that...

    • Gram-negative

      Microscopic image of gram-negative Pseudomonas aeruginosa...

    • Diseases Caused by E. Coli
    • Characteristics of E. Coli
    • Virulence Factors of E.coli
    • Diagnostic Features of E.coli
    • Antimicrobial Susceptibility Testing
    E.coli is the second most important cause of Gram-negative rod sepsis
    Perinatal infection with E.coli (exposure of newborn to E.coli colonized in the birth canal of the mother during natural birth) is the predominant cause of neonatal meningitis
    Enterotoxigenic Escherichia coli (ETEC) is responsible for the traveler’s diarrhea(watery diarrhea)
    Gram stain: Escherichia coliis a straight gram-negative short rod or bacilli
    Escherichia coli cells are small rods 1.0-2.0 micrometers long, with a radius of about 0.5 micrometers. However, the size varies with the medium, and faster-growing cells are larger.
    E. coliis the most abundant facultative anaerobe in the colon and feces.
    The generation (doubling) time of Escherichia coliis 20 minutes.
    Pili:Helps in adherence of organisms to the cells of jejunum and ileum in case of intestinal tract infection; urinary tract epithelium in case of urinary tract infections.
    Endotoxin (lipopolysaccharide): Responsible for several features of gram-negative sepsis such as fever, hypotension, and disseminated intravascular coagulation (DIC).
    Exotoxinse.g. enterotoxin act on the cells of the jejunum and ileum to cause diarrhea. Other exotoxins are verotoxin, Shiga-like toxin, etc.
    Colony Morphology: E.coli ferments lactose and produces pink colonies on MacConkey Agar. Typical colonies of Escherichia coli on MacConkey agar will appear pink and shiny and have a diameter of 0.5...
    On EMB agar, E. coliproduces a characteristic green sheen.

    Perform the susceptibility test by a disc-diffusion method using standard methods described in the guidelines. To detect ESBL-producing E.coli, the isolate screened should be multidrug-resistant exhibiting resistance to at least one of the third-generation cephalosporins. Extended-spectrum ß-lactamases (ESBL) are a rapidly evolving group of ß-lacta...

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  3. Nov 18, 2022 · Escherichia coli is a type of bacteria that lives in many places in the environment, including the gastrointestinal system of humans and warm-blooded animals, where it is part of the gut ...

  4. Nov 11, 2022 · Escherichia coli is a type of bacteria that lives in many places in the environment, including the gastrointestinal system of humans and warm-blooded animals, where it is part of the gut microbiota. Some strains of E. coli can be administered as probiotics and are known to have a positive effect on host health. However, some strains can be pathogenic, causing intestinal and extraintestinal ...

  5. Jan 1, 2008 · E. coli is Gram-negative and its envelope has three layers: cytoplasmic membrane, peptidoglycan, and outer membrane. The peptidoglycan is rigid determining the rod shape. To a good approximation, the E. coli cell has hemispherical caps and a cylindrical section in between.

    • Galina Reshes, Sharon Vanounou, Itzhak Fishov, Mario Feingold
    • 10.1529/biophysj.107.104398
    • 2008
    • Biophys J. 2008 Jan 1; 94(1): 251-264.
  6. Dec 31, 2022 · Abstract. Escherichia coli have served as important model organisms for over a century—used to elucidate key aspects of genetics, evolution, molecular biology, and pathogenesis. However, defining which strains actually belong to this species is erratic and unstable due to shifts in the characters and criteria used to distinguish bacterial ...

  7. Mar 7, 2017 · The top panels represent example morphological phenotypes observed in genes from the Keio collection: wild-type E. coli K-12 BW25113 cells, Δ tatA long and chained cells, Δ holD very long cells, Δ pdxH long and occasionally chained cells, Δ rodZ round cells, and Δ envC long cells.