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Dec 23, 2021 · Mean platelet count was significantly low in patients with elevated liver enzymes as compared to patients with normal levels of liver enzymes [Platelet count (81033.7(59256.8) vs. 147967.7(44726.9) cu.mm; P≤0.0001 respectively] Patients with elevated SGOT (93.8%) and SGPT (81.2%) had a higher incidence of bleeding manifestations.
Oct 5, 2022 · Picture 2. Headache, eye pain (ie, pain with eye movement), and joint pain occur in 60 to 70 percent of cases [ 24 ]. Rash occurs in approximately half of cases; it is more common during primary infection than secondary infection. When present, rash generally occurs two to five days after the onset of fever [ 24 ].
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Dengue fever is more common in males than in females, and it disproportionately affects those under the age of 30. Only 81 (54.63%) of the total 149 individuals developed DF, but of those, 79 (74.4%) had normal ALT levels and 2 (4.26%) had elevated ALT levels. Among the 68 patients with DHF (45.64%), 41 (87.23%) had elevated ALT, while only 23 ...
- Patients
- Confirmation of Dengue Infection and Dengue Antibody Assays
- Determining Viral Loads in Serial Blood Samples
- Generating Standard Curves For Quantification of The Denv
- Assays For Determining The Liver Profile
- Quantitative Elisa For Detection of Cytokines
- Statistical Analysis
Fifty five adult patients with acute dengue infection were recruited from the Infectious Diseases Hospital of Sri Lanka in year 2015 from January to August, following informed written consent. Those who were known to have chronic liver disease, chronic renal disease, pregnant individuals, those on a steroid dose of >40 mg/day for over 7 days and th...
Acute dengue infection was confirmed in the serum samples using the NS1 early dengue ELISA (Panbio, Australia) and by determining viral loads (see below). In order to determine if the patients had a primary or secondary dengue infection, the commercial capture-IgM and IgG enzyme-linked immunosorbent assay (ELISA) (Panbio, Brisbane, Australia) was u...
As we wished to determine the changes in viral loads in patients with SD and non-SD during the course of the illness, RNA was extracted from all serial serum samples using QIAamp Viral RNA Mini Kit (Qiagen, USA) according to the manufacturer’s protocol. The RNA was reverse transcribed into cDNA in GeneAmp PCR system 9700 using High Capacity cDNA re...
For generation of standard curves the four DENVs were grown in C6/36 cell lines supplemented with L15 media at 28 ° C. The virus supernatants were harvested at day 7 following infection and were immediately used to infect vero81 cell lines to determine the infective virus particles by plaque assays. The plaque assay was carried out on vero81 monola...
Assessment of the liver profile was done on all serial blood samples on a daily basis, obtained from the time of recruitment to discharge from hospital. Liver function tests to assess liver cell damage: aspartate transaminase (AST), alanine transaminase (ALT); tests to assess synthetic functions: serum albumin; tests to assess conjugative functions...
IL-10 and IL-17 levels were determined in serial serum samples, which were collected daily from the time of admission to discharge, using the human IL-10 enzyme linked immunosorbent assay (ELISA) PRO kit (Mabtech, Sweden) and LEGEND MAX™ Human IL-17A ELISA Kit (BioLegend, USA). The ELISAs were performed and results were interpreted according to the...
Statistical analysis was performed using Graph PRISM version 6. Differences in the serial values of liver enzymes, viral loads and cytokine values in patients with NSD and SD were done using multiple unpaired t tests. Corrections for multiple comparisons were done using Holm-Sidak method and the statistical significant value was set at 0.05 (alpha)...
- Samitha Fernando, Ananda Wijewickrama, Laksiri Gomes, Chameera T. Punchihewa, S. D. P. Madusanka, Ha...
- 2016
Dec 23, 2021 · Conclusion: liver involvement in the form of elevated transaminases was found in 74.2% dengue patients. Serum glutamic-oxaloacetic transaminase and serum glutamic-pyruvic transaminase level increases with increase in dengue severity which is indicated by fall in platelet count as they are negatively correlated with each other.
The following laboratory tests should be performed: Complete blood cell (CBC) count. Metabolic panel. Serum protein and albumin levels. Liver panel. Coagulation profile and disseminated intravascular coagulation (DIC) panel. Characteristic findings in dengue fever are thrombocytopenia (platelet count < 100 x 10 9 /L), leukopenia, and mild-to ...
