Search results
Oct 16, 2020 · In freshly voided urine, the culture of ≥ 100 colonies of one type (number of bacteria is ≥ 10 5 cfu/mL) has usually been regarded as a cutoff for UTI (Table 1) [9,10]. If 10–100 colonies of one type are counted (number of bacteria is between 10 4 and 10 5 cfu/mL), the result should be evaluated according to the clinical status.
Nov 18, 2021 · In contrast to other E. coli pathotypes, UPEC does not possess a specific virulence profile, but its virulence genes are mainly associated with characteristics such as adherence, motility, iron capture, and toxigenicity.
- Manuel G Ballesteros-Monrreal, Margarita M P Arenas-Hernández, Edwin Barrios-Villa, Josue Juarez, Ma...
- 10.3390/microorganisms9112381
- 2021
- Microorganisms. 2021 Nov; 9(11): 2381.
People also ask
What are the phenotypes of E coli virulence?
How does E coli locate a division septum?
Is uropathogenic Escherichia coli a group-specific gene expression profile?
Jan 1, 2008 · E. coli is Gram-negative and its envelope has three layers: cytoplasmic membrane, peptidoglycan, and outer membrane. The peptidoglycan is rigid determining the rod shape. To a good approximation, the E. coli cell has hemispherical caps and a cylindrical section in between.
- Galina Reshes, Sharon Vanounou, Itzhak Fishov, Mario Feingold
- 10.1529/biophysj.107.104398
- 2008
- Biophys J. 2008 Jan 1; 94(1): 251-264.
Apr 15, 2024 · To determine and compare the virulence characteristics and pathogenicity of E. coli strains isolated from hospital-acquired UTI patients and community-acquired UTI patients, the presence of eight virulence genes, namely, cnf1, traT, ompT, hlyD, tsh, ibeA, sitA and malX, was investigated in E. coli isolates using specific primers and PCR method.
- Bacterial Strains
- Phylotyping and Virulence Genotyping
- Analysis of Biofilm Formation Capacity
- Hemolysin Production
- Antibiotic Susceptibility Testing
- Detection of ESBL
- Plasmid DNA Profile
- Statistical Analysis
A total of 190 clinical non-duplicate E. coli isolates were collected from midstream urines obtained by the clean-catch method. UTI was defined as >104 leukocytes per milliliter urine and the growth of a single pathogen of >105 CFU/mL from urine specimens . Strains were identified based on observing colonial morphology on EMB medium. Lactose-fermen...
Phylogenetic Analysis
All isolates were cultured in Luria–Bertani (LB) agar and incubated overnight at 37 °C. Genomic DNA was extracted from phenotypically confirmed E. coli isolates by a DNA extraction kit (GeneJET Genomic DNA Purification Kit, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions and used for subsequent polymerase chain reaction (PCR) analysis. E. coli ATCC 25922, E. coli ATCC 35150, K. pneumoniae ATCC 700603, and Staphylococcus aureusATCC 25923 were used as co...
Virulence Genotyping
Screening of nine virulence factors (VFs) including: two adhesin-encoding genes (fimH, PapAH), three protectins/serum survival-related gene (kpsMTII, ompT, traT), two iron acquisition/uptake systems-related genes (iutA, fyuA), one pathogenicity island marker (PAI), and one uropathogenic-specific protein (usp) were determined by PCR as previously described [20,21,22,23]. Strain 3462 was taken as positive control for usp, PAI, fyuA, and fimH sequences and the strain 4166 was used as positive co...
Congo Red Agar Method by Freeman et al.
Suspension of tested strains was inoculated onto a specially prepared solid medium brain–heart infusión broth (BHI) supplemented with 5% sucrose and Congo red. The medium was composed of BHI (37 g/L), sucrose (50 g/L), agar no. 1 (10 g/L), and Congo red stain (0.8 g/L). Congo red was prepared as concentrated aqueous solution and autoclaved at 121 °C for 15 min, separately from other medium constituents and was then added when the agar had cooled to 55 °C. Plates were inoculated and incubated...
Screening of Morphotypes
The morphotypes of each strain were determined by the morphology of the colonies after incubation for 24 h at 37 °C. All plates were visually examined and the morphotypes were categorized as follows: red, dry, and rough (rdar) indicating expression of curli fimbriae and cellulose; brown, dry, and rough (bdar) indicating expression of fimbriae but not cellulose; pink, dry, and rough (pdar) indicating expression of cellulose but not fimbriae; and smooth and white, dry, and rough (saw) indicatin...
Microtiter Plate Assay
A crystal violet staining method was employed to examine biofilm-forming abilities of the isolates with modifications. The isolates were inoculated into 1 mL LB broth and grown overnight at 37 °C with constant shaking. Overnight cultures were transferred to new culture medium (diluted by 1:100) and grown to OD 600 between 0.45 and 0.65 and for each strain assay, it was done in triplicate. Thirty microliters of bacteria in log phase growth were inoculated into 96-well polystyrene plates c...
The hemolytic capacity of the isolates was evaluated using the method proposed by Scheffer et al. , with some modifications. Cultures at a concentration of 6 × 108 cells were obtained. 0.1 mL of culture was incubated with 0.9 mL of a 2% sheep red blood cell suspension in buffer (20 mM CaCl2, 10 mM Tris and 140 mM NaCl, pH 7.4) for 30 min at 37 °C; ...
Bacterial susceptibility to antimicrobial agents was determined by the disk diffusion method on Mueller Hinton agar and interpreted according to Clinical Laboratory Standards Institute (CLSI) guidelines . The following antibiotics were used: Ampicilline (AM), Ampicillin/sulbactam (SAM), Cefotaxime (CTX), Nitrofurantoin (NIT), Amikacin (AMK), Ciprof...
(a) Phenotypic screening of ESBL Isolates was done for resistance to three oxyimino-cephalosporins: ceftazidime (30 μg), cefotaxime (30 μg), ceftriaxone (30 μg) and the monobactam: aztreonam (30 μg...(b) Phenotypic confirmatory method of ESBL ESBL production was detected by the double-disk synergy test (DDST) using clavulanic acid-amoxicillin (20/10 μg) and ceftazidime (30 μg), cefotaxime (30 μ...(c) Detection of CTX-M-1 ESBL gene by PCR Genomic DNA was performed as described elsewhere (see “Phylogenetic Analysis” section). The presence of beta-lactamase gene blaCTX-M-1 was detected by PCR...Plasmid DNA was extracted with a small-scale alkaline lysis with SDS method . Extracted plasmids were electrophoresed for 2 h in a horizontal 1% agarose gel with pH 8.0 and 0.5 X TAE buffer. The gels were stained with ethidium bromide 0.5 mg/mL for 20 min, and bands were visualized by UV transilluminator. Molecular weight marker Promega™ Lambda DNA...
Descriptive statistics were used to estimate the frequencies of occurrence of the study variables. Biofilm-forming abilities were compared using the Mann–Whitney U test. Chi-square was used to assess significant differences about the association between the phylogenetic groups, virulence genes, MDR, ESBL, biofilm, and hemolysin production. Fisher’s...
- Rosa Baldiris-Avila, Alfredo Montes-Robledo, Alfredo Montes-Robledo, Yaleyvis Buelvas-Montes
- 2020
Jan 11, 2022 · Article. Open access. Published: 11 January 2022. Transcriptional alterations in bladder epithelial cells in response to infection with different morphological states of uropathogenic...
Aug 14, 2017 · Comparative proteomics of uropathogenic Escherichia coli during growth in human urine identify UCA-like (UCL) fimbriae as an adherence factor involved in biofilm formation and binding to uroepithelial cells.
