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  1. The sensitivity of microscopy can be poor (60%) and confounded with false-positive results due to misidentification of macrophages as trophozoites, PMNs as cysts (especially when lobed nuclei of PMNs break apart), and other Entamoeba species (67, 72, 76, 80, 188) (Fig. (Fig.1; 1; Table Table1 1).

  2. Entamoeba coli, E. hartmanni, E. polecki, Endolimax nana, and Iodamoeba buetschlii are generally considered nonpathogenic and reside in the large intestine of the human host . Both cysts and trophozoites of these species are passed in stool and considered diagnostic . Cysts are typically found in formed stool, whereas trophozoites are typically ...

  3. The cyst of Entamoeba coli , shown at right, measures between 15 and 25 µm in diameter. The key identification feature is the number of nuclei. A mature cyst will have 8 nuclei; occasionally immature cysts with 2 nuclei will be seen. The image at right is an iodine wet-mount (1000x). The trophozoites of E. coli typically have a diameter of 20 ...

  4. Entamoeba coli: 10-35 µm Usual range, 15-25 µm. Usually spherical. Occasionally oval, triangular, or other shapes. 8 in mature cyst. Occasionally super-nucleated cysts with 16 or more are seen. Immature cysts with 2 or more occasionally seen. Peripheral chromatin present.

  5. Wet mount. Entamoeba histolytica and Entamoeba dispar are morphologically identical species. In bright-field microscopy, E. histolytica/E. dispar cysts are spherical and usually measure 12 to 15 μm (range may be 10 to 20 μm). A mature cyst has 4 nuclei while an immature cyst may contain only 1 to 3 nuclei. Peripheral chromatin is fine ...

  6. This trichrome-stained photomicrograph, depicted a mature, Entamoeba coli cyst, containing five, clearly visible nuclei, and a what may have been a sixth nucleus, which was outside the focal plane, and therefore, out of focus. The cyst also contained a visible chromatoid body.

  7. Morphology & Diagnosis. Entamoeba coli are detected in the feces during an ova & parasite examination. Trophozoite and cyst forms can be detected. As with all ameba the trophozoite and cyst forms are seen in liquid stool while cysts are generally only seen in formed stool. The trophozoites range in size between 15-50u but normally are 20-24u in ...

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