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Cysts of Entamoeba coli are usually spherical but may be elongated and measure 10–35 µm. Mature cysts typically have 8 nuclei but may have as many as 16 or more. Entamoeba coli is the only Entamoeba species found in humans that has more than four nuclei in the cyst stage. The nuclei may be seen in unstained as well as stained specimens.
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Aug 23, 2023 · This photomicrograph of an iron-hematoxylin-stained specimen, revealed the presence of a mature, Entamoeba coli cyst, which contained five visible nuclei, each with its eccentrically located karyosome, and peripherally located chromatin.
- Akhlema Haidar, Orlando De Jesus
- 2023/02/12
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How many nuclei does an Entamoeba cyst have?
How many nuclei does Entamoeba coli have?
Do Entamoeba coli trophozoites have a karyosome?
What is the difference between trophozoites and cysts in Entamoeba histolytica?
E. coli has cysts in size up to 10 to 35 micrometers, the shape is irregular, oval with a shell-like appearance that is more uniformed compared to E. histolytica, and has up to eight nuclei in the cyst compared to the four nuclei of E. histolytica.
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Immature cysts typically have a large glycogen vacuole and two nuclei. Mature cysts have eight nuclei, with hypernucleate forms having 16 or more nuclei. The presence of an Entamoeba cyst in human stool with more than four nuclei is pathognomonic for E. coli (Ash and Orihel, 2007).
Structure. The trophozoite is 10 to 60 µm in diameter, ameboid, actively motile, and often erythrophagocytic. In stained specimens, the nucleus has a central karyosome with finely beaded peripheral chromatin. The cyst form is rounded, 10 to 20 µm in diameter, with one to four nuclei showing the characteristic appearance.
In this focal plane, the smaller Entamoeba histolytica cyst (top), contained four nuclei, and two bluntly-tipped chromatoid bodies, while six nuclei could be detected in the larger Entamoeba coli cyst (bottom), which also contained two chromatoid bodies as well.
