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Cysts of Entamoeba coli are usually spherical but may be elongated and measure 10–35 µm. Mature cysts typically have 8 nuclei but may have as many as 16 or more. Entamoeba coli is the only Entamoeba species found in humans that has more than four nuclei in the cyst stage. The nuclei may be seen in unstained as well as stained specimens.
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This usually involves checking the cysts for the size, shape, and the number of nuclei. E. coli has cysts in size up to 10 to 35 micrometers, the shape is irregular, oval with a shell-like appearance that is more uniformed compared to E. histolytica, and has up to eight nuclei in the cyst compared to the four nuclei of E. histolytica.
- E. coli
- Entamoeba
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Aug 23, 2023 · This photomicrograph of an iron-hematoxylin-stained specimen, revealed the presence of a mature, Entamoeba coli cyst, which contained five visible nuclei, each with its eccentrically located karyosome, and peripherally located chromatin.
- Akhlema Haidar, Orlando De Jesus
- 2023/02/12
Entamoeba coli. The cyst of Entamoeba coli , shown at right, measures between 15 and 25 µm in diameter. The key identification feature is the number of nuclei. A mature cyst will have 8 nuclei; occasionally immature cysts with 2 nuclei will be seen. The image at right is an iodine wet-mount (1000x).
Immature cysts typically have a large glycogen vacuole and two nuclei. Mature cysts have eight nuclei, with hypernucleate forms having 16 or more nuclei. The presence of an Entamoeba cyst in human stool with more than four nuclei is pathognomonic for E. coli (Ash and Orihel, 2007).
Cysts of Entamoeba hartmanni are similar to those of E. histolytica but smaller, measuring 5-10 µm. Mature cysts contain four nuclei that possess a small, discrete centrally-located karyosome and evenly-distributed peripheral chromatin. Cysts may not be visible in unstained specimens.
Aug 23, 2023 · Mature Entamoeba coli Cyst. This trichrome-stained photomicrograph depicts a mature Entamoeba coli cyst containing five clearly visible nuclei and what may have been a sixth nucleus, which was outside the focal plane and, therefore, out of focus. The cyst also contained a visible chromatoid body.
