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Jul 14, 2014 · Abstract. Entamoeba histolytica, the causative agent of intestinal and extraintestinal amebiasis, is a common parasitic cause of significant morbidity and mortality in the developing countries. Hence, early detection and differentiation of pathogenic E. histolytica from nonpathogenic/commensal Entamoeba spp ( Entamoeba dispar/Entamoeba ...
- Progress in The Research on Diagnosis and Vaccines in Amebiasis
INTRODUCTION. Entameba histolytica the causative agent of...
- Entamoeba Bangladeshi Nov. Sp., Bangladesh
An antigen detection test (TechLab Inc. Blacksburg, VA, USA)...
- Progress in The Research on Diagnosis and Vaccines in Amebiasis
Apr 17, 2023 · The pathological range includes mucosal inflammation, thickening, ulcers, and necrosis, leading to perforation. Amoebic cysteine proteinases can also contribute to trophozoites' ability to suppress a host’s immune response by being able to cleave and inactivate anaphylatoxins C3a, C5a, IgA, and IgG. [2]
A PCR solution hybridization enzyme-linked immunoassay targeting extrachromosomal circular DNA from E. histolytica and E. dispar with specific primers and a biotin-conjugated probe was shown to be sensitive for detection and differentiation of the two Entamoeba species in clinical samples ( 7, 11, 199 ).
- R. Fotedar, D. Stark, N. Beebe, D. Marriott, J. T. Ellis, J. Harkness
- 10.1128/CMR.00004-07
- 2007
- Clin Microbiol Rev. 2007 Jul; 20(3): 511-532.
- Historical Background
- Morphology and Biology
- Epidemiology
- Amebiasis in The Mediterranean Countries
- Pathophysiology
- Clinical Picture
- Ameboma
- Laboratory Diagnosis
- Microscopy
- Culture
Ronald Elsdon Dew made a full comprehensive description of the history of amoebae in a review article published in 1969 . He mentioned that Lösch was the first to report the amoebic trophozoites in the stools of a Russian patient in St. Petersburg suffering from severe dysentery in 1875. Definite association of amoeba with disease was established b...
Entamoeba histolytica occurs in the following forms, the trophozoites, precyst, cyst, metacyst, and metacystic trophozoite. The trophozoite is about 10–60 μm in size with a clear finely granular cytoplasm and a spherical nucleus that shows aggregation of chromatin beads at the nuclear membrane and a centrally located karyosome. The trophozoite is t...
Amebiasis is responsible for around 100,000 deaths/year, mainly in Central and South America, Africa, and India, as well as for a significant rate of morbidity manifested as invasive intestinal or extraintestinal disease . Infection with E. histolytica has been estimated to be as high as 50% in some developing countries as South and Central America...
The Mediterranean countries cover parts of the three old continents Africa, Asia, and Europe with an area of 2,085,292 km2 “(https://en.wikipedia.org/wiki/Mediterranean_Basin).” Its climate is generally moderate; however, it differs according to many factors as topography, plant cover, and rain fall. These differences plus political and financial s...
Amoebiasis is thought to be one of the most commonly known parasitic diseases affecting millions of people worldwide [57, 58]. Many of the cases have been discovered accidentally during a routine stool analysis and unfortunately many of them were given unneeded treatment for ameba. Those cases infected with the organism without symptoms were called...
Infection may be asymptomatic in most of the cases; however, severe fulminating disease may occur after an incubation period of 7–28 days from exposure to infection. Mild symptoms may be in the form of abdominal cramps, diarrhea with passage of 3–8 soft stools/day, or passage of stool with mucus and occasional blood. There may be fatigue, excessive...
It is one of the rare complications of colonic infection with pathogenic E. histolytica. It occurs as a result of deep invasion of the wall of the colon by invasive E. histolytica trophozoites with extensive formation of granulation tissue and the development of tumor-like mass. The most common sites of ameboma are the cecum and ascending colon. It...
Amebiasis is diagnosed in the laboratory by detecting the parasite in specimens or by an immunologic or a molecular technique [79,80,81,82,83]. In case of invasive intestinal amebiasis, the blood picture in 80% of cases may show leukocytosis without eosinophilia, elevation of erythrocytic sedimentation rate (ESR), elevation of serum alkaline phosph...
Identification of haematophagous trophozoites in fresh stool smears is the primary step for the identification of intestinal amebiasis and is an indication of infection [1•]. Because of irregular output of the parasite stages in stool, a single stool analysis is not usually positive. To further improve the sensitivity of the microscopic examination...
Culture is a method of diagnosis, however it is not easy to perform and less sensitive than microscopic examination with a success rate of 50–70% [1•]. Xenic cultivation, first introduced in 1925, is defined as the growth of the parasite in the presence of an undefined flora. This technique is still in use today utilizing the modified Locke egg or ...
- Nadia A. El-Dib
- naeldib@hotmail.com
- 2017
Aug 19, 2019 · Introduction. Entamoeba histolytica is a protozoan parasite of humans, and causative agent of amoebiasis. The parasite exists in two forms; an infective, non-dividing cyst stage and an actively dividing, invasive trophozoite. The dormant, non-motile cyst, upon ingestion by the host, gets converted to the actively dividing trophozoite in the colon.
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Jan 1, 2014 · Abstract. This chapter discusses Entamoeba histolytica, basic microbiology, natural history and life cycle, clinical features, pathogenicity, virulence and causation, treatment, methods of detection, survival and detection in the environment and in water, a critical review of the epidemiology and, finally, risk assessment.
Entamoeba histolytica / cytology. Entamoeba histolytica / isolation & purification* Feces / parasitology* Humans. Staining and Labeling / methods* Time Factors. Trophozoites / classification* Trophozoites / cytology* Entamoeba histolytica causes about 50 million infections worldwide with a death rate of over 100,000 annually.
