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  1. Jul 14, 2014 · There are several factors affecting the detection of Entamoeba spp by microscopy which are lack of adequate training in microscopy, delay in delivery of the sample to the laboratory leading to degradation/death of active trophozoite forms, difficulty in differentiation of cyst with degenerated polymorphonuclear cells particularly the mature ...

  2. A PCR solution hybridization enzyme-linked immunoassay targeting extrachromosomal circular DNA from E. histolytica and E. dispar with specific primers and a biotin-conjugated probe was shown to be sensitive for detection and differentiation of the two Entamoeba species in clinical samples ( 7, 11, 199 ).

    • R. Fotedar, D. Stark, N. Beebe, D. Marriott, J. T. Ellis, J. Harkness
    • 10.1128/CMR.00004-07
    • 2007
    • Clin Microbiol Rev. 2007 Jul; 20(3): 511-532.
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    • Causal Agents
    • Life Cycle
    • Geographic Distribution

    Several protozoan species in the genus Entamoeba colonize humans, but not all of them are associated with disease. Entamoeba histolytica is well recognized as a pathogenic ameba, associated with intestinal and extraintestinal infections. Other morphologically-identical Entamoeba spp., including E. dispar, E. moshkovskii, and E. bangladeshi, are gen...

    Cysts and trophozoites are passed in feces . Cysts are typically found in formed stool, whereas trophozoites are typically found in diarrheal stool. Infection with Entamoeba histolytica (and E.dispar) occurs via ingestion of mature cysts from fecally contaminated food, water, or hands. Exposure to infectious cysts and trophozoites in fecal matter d...

    Pathogenic Entamoeba species occur worldwide and are frequently recovered from fresh water contaminated with human feces. The majority of amebiasis cases occur in developing countries. In industrialized countries, risk groups include men who have sex with men, travelers, recent immigrants, immunocompromised persons, and institutionalized population...

  4. Entamoeba histolytica trophozoites observed under the microscope stain with methylene blue (Observe that the cells did not accept the stain since they were still alive at the time the...

  5. Feb 13, 2017 · The nonpathogenic ameba feeds on bacterial microbiota and intestinal contents. In fresh-stool examined under the microscope, the trophozoite moves actively by a finger-like protrusion of the ectoplasm “pseudopodium,” into which the cytoplasm is pulled moving the whole body of the organism in its direction.

    • Nadia A. El-Dib
    • naeldib@hotmail.com
    • 2017
  6. Dec 19, 2013 · Entamoeba histolytica is the pathogenic amoeba responsible for amoebiasis, an infectious disease targeting human tissues. Amoebiasis arises when virulent trophozoites start to destroy the muco-epithelial barrier by first crossing the mucus, then killing host cells, triggering inflammation and subsequently causing dysentery.

  7. May 26, 2016 · A proteomic study of E. histolytica trophozoites, cysts, and in vitro -produced CLS is reported herein to determine the nature of CLS, widen our knowledge on the cyst stage, and identify possible proteins and pathways involved in the encystment process.

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