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  1. Jul 14, 2014 · There are several factors affecting the detection of Entamoeba spp by microscopy which are lack of adequate training in microscopy, delay in delivery of the sample to the laboratory leading to degradation/death of active trophozoite forms, difficulty in differentiation of cyst with degenerated polymorphonuclear cells particularly the mature ...

  2. A PCR solution hybridization enzyme-linked immunoassay targeting extrachromosomal circular DNA from E. histolytica and E. dispar with specific primers and a biotin-conjugated probe was shown to be sensitive for detection and differentiation of the two Entamoeba species in clinical samples ( 7, 11, 199 ).

    • R. Fotedar, D. Stark, N. Beebe, D. Marriott, J. T. Ellis, J. Harkness
    • 10.1128/CMR.00004-07
    • 2007
    • Clin Microbiol Rev. 2007 Jul; 20(3): 511-532.
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    • Mode of Transmission
    • Life Cycle of Entamoeba histolytica
    • Diseases
    • Clinical Findings
    • Laboratory Diagnosis

    Feco-oral route, via the ingestion of contaminated food or water containing mature quadrinucleate cyst of Entamoeba histolytica. Trophozoites if ingested would not surviveexposure to the gastric environment. Infective form: Mature quadrinucleate cyst; it is spherical in shapewith a refractile wall Geographical distribution: Worldwide, more common i...

    Infection by Entamoeba histolytica occurs by the ingestion of mature quadrinucleate cysts in fecally contaminated food, water, or hands. The quadrinucleate cyst is resistant to the gastric environmentand passes unaltered through the stomach 1. When the cyst of E.histolytica reaches caecum or lower part of ileum excystation occurs and anamoeba with ...

    Non-invasive infection: In many cases, the trophozoites remain confined to the intestinal lumen of individuals who are thus asymptomatic carriers and cysts passers.
    Intestinal disease: In some patients, the trophozoites invade the intestinal mucosa,
    Extra-intestinal disease: through the bloodstream, trophozoites invade extraintestinal sites such as the liver, brain, and lungs, with resultant pathologic manifestations.
    Acute intestinal amebiasis
    Chronic amebiasis: low-grade symptoms such as occasional diarrhea, weight loss, and fatigue also occurs.
    Roughly 90% of infected individuals have an asymptomatic infection but they may be carriers.
    Ameboma, a granulomatous lesion may form in the cecal or rectosigmoid areas of the colon in some patients. These lesions resemble an adenocarcinoma of the colon and must be distinguished from them.

    Diagnosis of intestinal amebiasis rests on finding either trophozoites in diarrheal stools or cysts in formed stools. Diarrheal stools should be examined within one hour of collection to see the ameboid motility of the trophozoite. The trophozoite characteristically contains ingested red blood cells. Characteristics of Stool 1. Macroscopic appearan...

  4. Apr 17, 2023 · There are two forms E. histolytica can take: the cyst form, which can survive in the environment for a prolonged period, and the trophozoite stage, which is the active and invasive form. After the ingestion of the cyst form, trophozoites can be formed, which can invade and penetrate intestinal mucosa destroying epithelial cells and inflammatory ...

  5. Cysts are typically found in formed stool, whereas trophozoites are typically found in diarrheal stool. Infection with Entamoeba histolytica (and E.dispar) occurs via ingestion of mature cysts from fecally contaminated food, water, or hands. Exposure to infectious cysts and trophozoites in fecal matter during sexual contact may also occur.

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  6. Dec 19, 2013 · Entamoeba histolytica is the pathogenic amoeba responsible for amoebiasis, an infectious disease targeting human tissues. Amoebiasis arises when virulent trophozoites start to destroy the muco-epithelial barrier by first crossing the mucus, then killing host cells, triggering inflammation and subsequently causing dysentery.

  7. Entamoeba histolytica is a unicellular, protozoon parasite of humans. It moves by a jelly-like tongue-like protrusion of the cytoplasm “pseudopodium. Infection with E. histolytica may. ” be the cause of a variety of symptoms, beginning from no symptoms to severe fulminating intestinal and/or life-threatening extraintestinal disease.

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