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  1. Entamoeba histolytica trophozoites observed under the microscope stain with methylene blue (Observe that the cells did not accept the stain since they were still alive at the time the picture was ...

  2. Entamoeba histolytica . and . Entamoeba dispar. are morphologically identical species. In bright-field microscopy, E. histolytica / E. dispar. cysts are spherical and usually measure 12 to 15 µm (range may be 10 to 20 µm). A mature cyst has 4 nuclei while an immature cyst may contain only 1 to 3 nuclei.

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    Several protozoan species in the genus Entamoeba colonize humans, but not all of them are associated with disease. Entamoeba histolytica is well recognized as a pathogenic ameba, associated with intestinal and extraintestinal infections. Other morphologically-identical Entamoeba spp., including E. dispar, E. moshkovskii, and E. bangladeshi, are generally not associated with disease although investigations into pathogenic potential are ongoing. While the discussed species are morphologically-identical, E. histolytica may be observed with ingested red blood cells (erythrophagocytosis); E. dispar may occasionally be seen with ingested erythrocytes as well, although its capacity for erythrophagocytosis is much less than that of E. histolytica. Non-pathogenic amebae (e.g. Endolimax nana, Iodamoeba buetschlii, other Entamoeba species) are important because they may be confused with E. histolyticain diagnostic investigations.

    Cysts and trophozoites are passed in feces . Cysts are typically found in formed stool, whereas trophozoites are typically found in diarrheal stool. Infection with Entamoeba histolytica (and E.dispar) occurs via ingestion of mature cysts from fecally contaminated food, water, or hands. Exposure to infectious cysts and trophozoites in fecal matter during sexual contact may also occur. Excystation occurs in the small intestine and trophozoites are released, which migrate to the large intestine. Trophozoites may remain confined to the intestinal lumen (A: noninvasive infection) with individuals continuing to pass cysts in their stool (asymptomatic carriers). Trophozoites can invade the intestinal mucosa (B: intestinal disease), or blood vessels, reaching extraintestinal sites such as the liver, brain, and lungs (C: extraintestinal disease). Trophozoites multiply by binary fission and produce cysts , and both stages are passed in the feces . Cysts can survive days to weeks in the extern...

    Pathogenic Entamoeba species occur worldwide and are frequently recovered from fresh water contaminated with human feces. The majority of amebiasis cases occur in developing countries. In industrialized countries, risk groups include men who have sex with men, travelers, recent immigrants, immunocompromised persons, and institutionalized populations.

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  4. G: Trophozoite of E. histolytica/E. dispar, measuring approximately 16.7 µm, stained with trichrome. The image was taken at 1000× magnification and contributed by the Kansas Department of Health and Environment. H: Trophozoite of E. histolytica. The specimen was preserved in poly-vinyl alcohol (PVA) and stained in trichrome.

  5. lungs, brain, spleen and skin. Pathology: Bacteria may enhance pathogenecity. Man is the reservoir of infection. Infections occur by 4 nucleated cysts. Entamoeba histolytica produces dysentery with frequent passing of stools mixed with mucus and blood. Intestinal lesions are acute amebic dysentery and chronic intestinal amebiasis.

  6. Entamoeba histolytica is an anaerobic parasitic amoebozoan, part of the genus Entamoeba. Predominantly infecting humans and other primates causing amoebiasis...

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