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  1. Dec 2, 2018 · 1. Introduction. Amoebiasis, or amoebic dysentery, is a term used to describe an infection caused by the protozoan Entamoeba histolytica [].Most infections are asymptomatic, but invasive intestinal disease may occur manifesting with several weeks of cramping, abdominal pain, watery or bloody diarrhea, and weight loss [].

  2. Entamoeba histolytica is the causative agent of amebiasis, a disease that is a major source of morbidity and mortality in the developing world. The potent cytotoxic activity of the parasite appears to underlie disease pathogenesis, although the mechanism is unknown. Recently, progress has been made in determining that the parasite activates apoptosis in target cells and some putative effectors ...

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  4. Apr 17, 2023 · Entamoeba histolytica is a protozoan that causes intestinal amebiasis as well as extraintestinal manifestations. Although 90 percent of E. histolytica infections are asymptomatic, nearly 50 million people become symptomatic, with about 100,000 deaths yearly.[1] Amebic infections are more prevalent in countries with lower socioeconomic conditions.[2]

  5. This lifecycle gives rise to the general way of how Entamoeba species form. This parasite has one large nucleus with a thick membrane surrounding the nucleus. There are many chromatin inside the nucleus, and one large, irregular-shaped karyosome. The chromatin is clumped, and uneven in disperse inside the nucleus.

  6. Mature Entamoeba histolytica/Entamoeba dispar cysts have 4 nuclei that characteristically have centrally located karyosomes and fine, uniformly distributed peripheral chromatin. Cysts usually measure 12 to 15 µm.

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  7. Apr 9, 2014 · For experiments with Caco-2 cells, CMFDA (Invitrogen) was used at 5 μM at 37 °C for 15 min; cells were then washed and incubated in fresh M199s at 37 °C for 10 min. Caco-2 cells were then ...

  8. Entamoeba histolytica occurs in the following forms, the tro-phozoites,precyst,cyst,metacyst,andmetacystictrophozoite. The trophozoite is about 1060 μm in size with a clear finely granular cytoplasm and a spherical nucleus that shows aggre-gation of chromatin beads at the nuclear membrane and a centrally located karyosome.

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