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Microscopy of the cysts
- Endolimax nana is diagnosed based on the microscopy of the cysts, involving concentration procedures (usually formalin-based) and different stains before the analysis is initiated. The cysts of Endolimax nana appear gibbous when the fecal concentrate is stained with iodine.
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Nov 4, 2015 · In addition, the development of diagnostic primers will allow Endolimax to be detected with high sensitivity using fecal DNAs and distinguished easily from other amebae. The clinical significance of Endolimax is still an unresolved issue. Prior exposure (immunity), parasite load, and genetic variability might influence clinical presentation.
Entamoeba coli, E. hartmanni, E. polecki, Endolimax nana, and Iodamoeba buetschlii are generally considered nonpathogenic and reside in the lumen of the large intestine in the human host. Both cysts and trophozoites of these species are passed in stool and are considered diagnostic .
Nov 4, 2015 · Abstract. Of the so-called nonpathogenic intestinal protozoa, Endolimax nana belongs to the ones least well described. Most data on E. nana have emerged from general surveys of intestinal parasites in selected cohorts and mostly in the absence of any particular focus on Endolimax. Hence, the genus of Endolimax remains largely unexplored in ...
- Study Design
- Study Population and Sample
- Data Collection
- Questionnaire
- Stool Sample Collection and Laboratory Methods
- Combined Microscopy Technique
- QPCR
- Data Analysis
A cross-sectional, community-based study, in which participants answered a questionnaire and provided one or two stool samples, was conducted between May 2015 and July 2016 in Bogotá. This study focused on pregnant women living in poor residential areas in three districts of Bogotá (Fig. 1). These communities were selected because they have a major...
Colombia’s health care system is organized into three levels of attention with primary care units (Unidad Primaria de Atención or UPA) within a basic level of health care and secondary and tertiary care hospitals. In Bogotá, antenatal care in public health settings is provided in UPAs with high-risk cases being referred. The study’s target populati...
With respect to national data protection regulations, it was not possible to access patient databases directly to establish a sample frame of pregnant women who attended the primary care units within the three districts. Therefore, from May 2015 to July 2016, research assistants proactively invited pregnant women to participate in the study. Fieldw...
The interviewer-administered questionnaire was adopted from the Demographic and Health Surveys (DHS) to assess sociodemographic conditions as implemented by ICF International (Fairfax, VA, USA) . The items included were age, occupation, education level, civil status, health insurance coverage, monthly income, household conditions, water availabilit...
All study participants received stool sample containers and standard instructions on proper and safe collection and preservation of the samples. By agreement, research assistants contacted participants by phone to insure that, once the sample was collected and stored in proper conditions (inside the fridge or in a dark low-temperature home location...
The detection techniques selected in this study were based on diagnostic performance, methodological availability, feasibility, and cost-effectiveness . In this study, each stool sample was analyzed by a so-called “combined microscopy technique”, which included sample analysis by direct wet mount microscopy and by a formol–ether concentration metho...
In this study, a subgroup of 48 stool samples was subsequently analyzed by qPCR for comparison with the results obtained by standard microscopy detection techniques. These samples originated from participants residing in Ciudad Bolívar district and were selected prospectively and chronologically by order of arrival at the laboratory. For financial ...
All data were double entered and controlled for errors. Data analysis was performed using IBM SPSS Statistics version 24 software (Armonk, NY, USA). A comparison of sociodemographic characteristics was performed between the 210 pregnant women who answered the questionnaire only and the 340 participants who also provided a stool sample. Laboratory r...
- Angela Fernanda Espinosa Aranzales, Angela Fernanda Espinosa Aranzales, Katja Radon, Guenter Froesch...
- 2018
Jan 1, 2016 · Abstract and Figures. Of the so-called nonpathogenic intestinal protozoa, Endolimax nana belongs to the ones least well described. Most data on E. nana have emerged from general surveys of ...
May 28, 2014 · There have been several studies that have suggested that pathogenicity may be subtype related. Metronidazole is the most widely accepted treatment for Blastocystis but several cases of treatment failure and resistance have been described. Other treatment options which have been suggested include paromomycin and trimethroprim- sulfamethoxazole.
Endolimax nana. Endolimax nana ( Fig. 4-1) is the smallest of the intestine-dwelling amoebae infecting humans, its trophozoite averaging only 8 μm in diameter (range, 6-15 μm). The trophozoite lives in the host’s colon and is generally considered to be nonpathogenic. According to some surveys, prevalence may be as high as 30% in some ...
