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1. www.cdc.gov › dengue › hcpMolecular Tests for Dengue Virus | Dengue | CDC

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   May 14, 2024 · Dengue virus RNA can usually be detected by nucleic acid amplification tests (NAAT) for the first 7 days of illness. A positive NAAT (e.g. RT-PCR) result confirms current dengue virus infection. A negative NAAT result does not rule out infection. People should also be tested for the presence of IgM antibodies against dengue virus to determine ...

2. www.cdc.gov › dengue › healthcare-providersMolecular Tests for Dengue Virus | Dengue | CDC

   www.cdc.gov › dengue › healthcare-providers

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   Jun 13, 2019 · June 14, 2019, Morbidity and Mortality Weekly Report: Dengue and Zika virus diagnostic testing for patients with a clinically compatible illness and risk for infection with both viruses For symptomatic persons with dengue virus infection, dengue virus RNA can usually be detected by molecular tests for the first 1-7 days in the course of illness.

3. People also ask

   How is a dengue virus diagnosed in a laboratory?

   • Laboratory diagnosis methods for confirming dengue virus infection may involve detection of the virus, viral nucleic acid, antigens or antibodies, or a combination of these techniques. After the onset of illness, the virus can be detected in serum, plasma, circulating blood cells and other tissues for 4–5 days.

   LABORATORY DIAGNOSIS AND DIAGNOSTIC TESTS - Dengue - NCBI B…

   www.ncbi.nlm.nih.gov/books/NBK143156/

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   Can dengue virus RNA be detected by a molecular test?

   • For symptomatic persons with dengue virus infection, dengue virus RNA can usually be detected by molecular tests for the first 1-7 days in the course of illness. What is the test? A NAAT is a generic term referring to molecular tests used to detect viral genomic material.

   Molecular Tests for Dengue Virus | Dengue | CDC

   www.cdc.gov/dengue/healthcare-providers/testing/molecular-tests/index.html

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   What is the full protocol for a dengue assay?

   • The full protocol is detailed in the supplementary methods. To ensure universal application of the assay to samples isolated worldwide, primers were designed against the conserved regions from alignments of serotype-specific dengue viruses isolated worldwide from 2005 to 2018.

   Single molecule, near full-length genome sequencing of dengue virus

   www.nature.com/articles/s41598-020-75374-1

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4. www.cdc.gov › dengue › healthcare-providersTesting Guidance | Dengue | CDC

   www.cdc.gov › dengue › healthcare-providers

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   • Acute Phase: Initial 1-7 Days After Symptom Onset
   • Convalescent Phase: >7 Days Post Symptom Onset
   • Testing to Differentiate Dengue from Other Flaviviruses
   • Interpreting Test Results

   The initial 1-7 days after symptom onset are referred to as the acute phase of dengue.

   During this period, dengue virus is typically present in blood or blood-derived fluids such as serum or plasma.

   Dengue virus RNA can be detected with molecular tests.

   The non-structural protein NS1 is a dengue virus protein that also can be detected using some commercial tests.

   See full list on cdc.gov

   The period beyond 7 days following symptom onset is referred to as the convalescent phase of dengue.

   Patients with negative NAAT or NS1 test results and negative IgM antibody tests from the first 7 days of illness should have a convalescent sample tested for IgM antibody test.

   During the convalescent phase, IgM antibodies are usually present and can be reliably detected by an IgM antibody test.

   IgM antibodies against dengue virus can remain detectable for 3 months or longer after infection.

   See full list on cdc.gov

   Special considerations: 1. Cross reactivity: Cross reactivity is a limitation of dengue serologic tests. Serologic tests to detect antibodies against other flaviviruses such Japanese encephalitis, St. Louis encephalitis, West Nile, yellow fever, and Zika viruses may cross react with dengue viruses. This limitation must be considered for patients wh...

   See full list on cdc.gov

   If a NAAT or NS1 test is positive for dengue, a current dengue diagnosis is confirmed.

   If the NAAT result is negative and the IgM antibody test is positive, the laboratory diagnosis is presumptive dengue virus infection.

   See full list on cdc.gov

5. www.ncbi.nlm.nih.gov › books › NBK143156LABORATORY DIAGNOSIS AND DIAGNOSTIC TESTS - Dengue - NCBI ...

   www.ncbi.nlm.nih.gov › books › NBK143156

   Laboratory diagnosis methods for confirming dengue virus infection may involve detection of the virus, viral nucleic acid, antigens or antibodies, or a combination of these techniques. After the onset of illness, the virus can be detected in serum, plasma, circulating blood cells and other tissues for 4–5 days.

   • Published: 2009

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7. news.mayocliniclabs.com › 2020/01/06 › dengue-virusDengue Virus: A Diagnostic Testing Update - Insights

   news.mayocliniclabs.com › 2020/01/06 › dengue-virus

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   Jan 6, 2020 · Overview. To begin, dengue virus is a member of the flavivirus family of viruses, which also include West Nile virus, St. Louis encephalitis virus, and yellow fever virus, among others. These are all enveloped, positive sense, single-stranded RNA viruses. The dengue virus complex is primarily comprised of four different dengue virus serotypes ...

8. www.nature.com › articles › s41598/020/75374-1Single molecule, near full-length genome sequencing of dengue ...

   www.nature.com › articles › s41598/020/75374-1

   Oct 23, 2020 · Dengue is a major public health problem with nearly half the global population at risk of infection 1,2.The incidence of dengue has increased by more than 30-fold in the last 50 years, and the ...

9. bmcgenomics.biomedcentral.com › articles › 10Structural landscape of the complete genomes of dengue virus ...

   bmcgenomics.biomedcentral.com › articles › 10

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   May 17, 2021 · Background With more than 300 million potentially infected people every year, and with the expanded habitat of mosquitoes due to climate change, Dengue virus (DENV) cannot be considered anymore only a tropical disease. The RNA secondary structure is a functional characteristic of RNA viruses, and together with the accumulated high-throughput sequencing data could provide general insights ...