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  1. Alfonso, Prince of Asturias. House. Aviz. Father. John, Constable of Portugal. Mother. Isabel of Barcelos. Isabella of Portugal ( Isabel in Portuguese and Spanish) (1428 – 15 August 1496) was Queen of Castile and León as the second wife of King John II. She was the mother of Queen Isabella I of Castile .

  2. During Charles' absences from Spain in 1529-1532 and 1535-1539, Isabella served as his regent. They had six children, of whom the first, third and fifth survived to adulthood. During one of Charles' absences, Isabella died after giving birth to her sixth child, a stillbirth. She was buried at Granada.

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  4. The daughter of Manuel I of Portugal and his second wife, Queen Maria of Castile , Isabella of Portugal was born in Lisbon on October 24, 1503. Her mother, a daughter of the Spanish monarchs, Ferdinand and Isabella I , was "very honest, devout, and charitable" and punished her children "when they deserved it without pardoning any of them."

  5. Isabella of Castile. Isabella of Castile. Isabella of Castile (April 22, 1451 – November 26, 1504) was queen of Castile and Aragon. Together with her husband, Ferdinand II of Aragon, their reign was a turning point for the Iberian Peninsula. The marriage of Isabella and Ferdinand joined the kingdoms of Castile and Aragon.

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