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Loop-mediated isothermal amplification (LAMP) is a single-tube technique for the amplification of DNA [2] for diagnostic purposes and a low-cost alternative to detect certain diseases. [3] LAMP is an isothermal nucleic acid amplification technique.
Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a one step nucleic acid amplification method to multiply specific sequences of RNA. It is used to diagnose infectious disease caused by RNA viruses .
Kerosene lamp, using kerosene as a fuel. Electric lamp, or light bulb, a replaceable component that produces light from electricity. Light fixture, or light fitting or luminaire, is an electrical device containing an electric lamp that provides illumination.
Apr 24, 2008 · Loop-mediated isothermal amplification (LAMP) is a simple, rapid, specific and cost-effective nucleic acid amplification method when compared to PCR, nucleic acid sequence-based...
- Norihiro Tomita, Yasuyoshi Mori, Hidetoshi Kanda, Tsugunori Notomi
- 2008
Jun 17, 2014 · LAMP is an isothermal amplification method designed to detect a target nucleic acid without requiring sophisticated equipment. LAMP provides high sensitivity (to fg or <10 copies of target) but with rapid results: reactions can be performed in as little as 5–10 minutes.
Loop-mediated isothermal amplification (LAMP) is well known for its robust and highly sensitive and specific amplification of target DNA, which is achieved by utilizing up to six primers.
Loop-mediated isothermal amplification (LAMP) uses 4-6 primers recognizing 6-8 distinct regions of target DNA. A strand-displacing DNA polymerase initiates synthesis and 2 of the primers form loop structures to facilitate subsequent rounds of amplification.
