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3 days ago · The research teams of Brandt Eichman, Professor and Chair of Biological Sciences and Professor of Biochemistry, and Walter Chazin, Professor of Biochemistry, worked together to understand how DNA replication is initiated in eukaryotes. Using Vanderbilt’s state-of-the-art instrumentation in the Center for Structural Biology’s Cryo-Electron Microscopy Facility, Eichman, Chazin, and their ...
2 days ago · Fig. 1: Nucleic acid-sensing activity after CFC. Fig. 2: DNA damage and DDR after CFC. Fig. 4: Single-cell changes of gene expression after neuron-specific deletion of hippocampal Tlr9. In summary ...
1 day ago · Primases are crucial enzymes for DNA replication, as they synthesize a short primer required for initiating DNA replication. We herein present time-resolved nuclear magnetic resonance (NMR) spectroscopy in solution and in the solid state to study the initial dinucleotide formation reaction of archaeal pRN1 primase. Our findings show that the helix-bundle domain (HBD) of pRN1 primase prepares ...
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Why do DNA polymerases make copies of DNA polynucleotide chains?
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4 days ago · Initiation of eukaryotic DNA replication is the first stage of DNA synthesis where the DNA double helix is unwound and an initial priming event by DNA polymerase α occurs on the leading strand. The priming event on the lagging strand establishes a replication fork.
4 days ago · Deoxyribonucleic acid (/ d iː ˈ ɒ k s ɪ ˌ r aɪ b oʊ nj uː ˌ k l iː ɪ k,-ˌ k l eɪ-/ ⓘ; DNA) is a polymer composed of two polynucleotide chains that coil around each other to form a double helix. The polymer carries genetic instructions for the development, functioning, growth and reproduction of all known organisms and many viruses.
6 days ago · DNA sequence of a gene is used to predict the mRNA sequence, and the genetic code is used to predict the amino acid sequence in return. What is an Intron? Non-coding sequence of DNA within a gene that is removed by RNA splicing in the nucleus, leaving an mRNA that is translated in the cytoplasm.
3 days ago · Library preparation is the step of an NGS workflow in which DNA or RNA molecules are processed and prepared for sequencing. Poorly prepared NGS libraries can result in low-quality sequences, failed sequencing, or inaccurate results. Depending on the type of sequencing being performed (e.g., whole genome sequencing, whole transcriptome ...