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The first step in DNA synthesis is to attach a protected deoxynucleoside to a silica (SiO 2) support by an ester linkage to the 3′ –OH group of the deoxynucleoside. Both the 5′ –OH group on the sugar and free –NH 2 groups on the heterocyclic bases must be protected.
Dec 27, 2023 · DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology that is used to determine the order of the four bases: adenine , guanine , cytosine , and thymine .
Apr 29, 2024 · The key steps in DNA synthesis include: Initiation: The process begins at specific sites on the DNA molecule called origins of replication. Enzymes called helicases unwind and separate the DNA strands, creating a replication fork. Primer synthesis: Primers, short RNA sequences, are synthesized by an enzyme called primase.
Jan 15, 2023 · describe, briefly, the steps required for chemical synthesis of DNA segments. DNA must be synthesized to study genes, the sequence of genomes, and many other studies. This occurs in two fashions, by polymerase chain reaction (PCR) and chemical synthesis.
The first step in DNA synthesis is to attach a protected deoxynucleoside to a silica (SiO 2) support by an ester linkage to the 3′ –OH group of the deoxynucleoside. Both the 5′ –OH group on the sugar and free –NH 2 groups on the heterocyclic bases must be protected.
Jan 27, 2024 · Principle. DNA synthesis led by the polymerase adds labeled nucleotides during each round of sequencing. Each nucleotide is added to its complementary part, read (imaged), recorded and enzymatically degraded to prepare the template to add the next nucleotide.
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This enzyme-powered copying process replicates the overlapping oligonucleotide strands into a single continuous gene, and also produces a partner sequence, forming enough double-stranded ...
