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  1. 8 hours ago · Demonstrating their technique in mouse embryonic stem cells, the researchers found that they could calculate approximately when a particular region starts to be transcribed, based on the length of the RNA strand and the speed of the polymerase (the enzyme responsible for transcription) — that is, how far the polymerase transcribes per second.

  2. en.wikipedia.org › wiki › BRCA1BRCA1 - Wikipedia

    8 hours ago · BRCA1 was shown to co-purify with the human RNA Polymerase II holoenzyme in HeLa extracts, implying it is a component of the holoenzyme. Later research, however, contradicted this assumption, instead showing that the predominant complex including BRCA1 in HeLa cells is a 2 megadalton complex containing SWI/SNF. [50]

  3. 8 hours ago · The genome of CjNRSV1 consists of three negative-sense single-stranded RNA segments, designated RNA1, RNA2, and RNA3, with lengths of 7252, 2401, and 1117 nucleotides, respectively. Notably, the 3'- and 5'-terminal regions of these RNA segments have identical sequences, and the termini are complementary to each other, a characteristic feature ...

  4. 8 hours ago · Real-time quantitative polymerase chain reaction (qPCR) was performed to determine the expression levels of genes in the extracted cDNA samples. For qPCR, specific primers were utilized, and the RealQ Plus 2x Master Mix Green (Ampliqon, Odense, Denmark) was employed, with the reaction carried out using the StepOne thermocycler (Applied ...

  5. 8 hours ago · RNA extraction, reverse transcription, and real-time polymerase chain reaction (PCR) Gene expression levels were analyzed using real-time PCR. The methods were based on our previous study [11, 12]. Briefly, the colon tissues were frozen at −80 °C in RNAlater™ solution (Thermo Fisher Scientific Inc.).

  6. 8 hours ago · The laboratory section will provide students with hands-on experimentations in major techniques in molecular biology such as DNA and RNA isolation, protein purification, DNA and protein electrophoresis, nucleic acid hybridization and polymerase chain reaction.

  7. 8 hours ago · CD Genomics performed the following procedures: genomic DNA extraction, polymerase chain reaction amplification of the V3-V4 region of 16 S rRNA gene using the primers 341 F = CCTACGGGNGGCWGCAG and 805R = GACTACHVGGGTATCTAATCC [17, 18] and purification and DNA library construction and sequencing.

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