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2 days ago · Steps. Keep primer’s melting temperature within 2°C. Use annealing temperature of between 3-5°C. Make primers range from 18-22 bases. Design with GC content of between 35 and 65%. Reduce GC repeats at the 3’ end of primers. Limit length of amplicon to about 140 base pairs.
1 day ago · Magnesium transports other electrolytes into the cell. Supports nerve signals and muscle contractions. Magnesium plays a multi-faceted role in heart health. It helps regulate heart rhythm, supports muscle function, and plays a role in the relaxation of blood vessels. Plus, it is critical in transporting potassium and calcium across the cell ...
5 hours ago · An original plasma chemical process initiated by microwave discharge in a mixture of metal and dielectric powders was applied to prepare specific materials, which consisted of microsized spherical particles of aluminum oxide covered with silver nanoparticles. The prepared materials are highly uniform in shape, size distribution, and composition. Their cytotoxicity was investigated using the ...
2 days ago · Reaction – The process of amplifying DNA primers depend on temperature and fewer proteins, while the replication process is often catalytic reaction that depends on fewer proteins and enzymes. Synthesis – RNA primers need Primase enzyme, whereas DNA primers are chemically synthesized.
3 days ago · This study introduces a novel class of hybrid compounds, namely, benzothieno[3,2’-f][1,3]oxazepines and their isomeric 2-oxo-2H-spiro[benzothiophene-3,3’-pyrrolines]. The synthetic strategy employs a three-component reaction and 1,4-Dipolar cycloaddition, yielding spiro and oxazepine compounds. Structural elucidation via NMR and MS analyses is complemented by X-ray crystallography and a ...
5 days ago · In principle, the organic matter in early Martian sediment could be due to abiotic reactions such as Fischer–Tropsch-type reactions 2,6 or electro-chemical reduction of CO 2 5,6,12, but none of ...
3 days ago · Sig: Saad Johar 1. Objective: This experiment is being performed to: Learn basic laboratory skills and the process of chemical synthesis. Perform limiting reactant stoichiometry calculations and analyze the result with the idea of significant figures. Theoretical background: This lab is performed to perform the synthesis of a coordination ...
