Search results
Our western blot protocol includes solutions and reagents, procedures, and useful links to guide you through your experiment. Reviewed December 14 2020. Western blotting is a technique that uses specific antibodies to identify proteins that have been separated based on size by gel electrophoresis.
General Protocol for Western Blotting Protein separation by gel electrophoresis 1. Load equal amounts of protein (20 μg) into the wells of a mini (8.6 x 6.7 cm) or midi (13.3 x 8.7 cm) format SDS-PAGE gel, along with molecular weight markers. 2. Run the gel for 5 min at 50 V. 3. Increase the voltage to 100–150 V to finish the run in about 1 hr.
- 96KB
- 2
Here, you can find a collection of western blot recipes for commonly used protein electrophoresis and western blot buffers and stock solutions, and general western blotting protocols for chemiluminescent and fluorescent detection to guide you through your experiment.
Western Blotting Protocol. Western blotting (WB) is widely used to analyze specific protein expression in cell or tissue extracts. At Cell Signaling Technology (CST) we understand that western blotting experiments are time consuming and that their success has a critical impact on your research progress.
Western blotting is a technique that uses specific antibodies to identify proteins that have been separated based on size by gel electrophoresis. Western blot guide. Download. PDF. Easily plan and optimize your western blot, and fluorescent western blot experiments with our complete guide.
Jan 24, 2022 · Western blot is a technique used to separate proteins by size followed by detection using antibodies specific to the protein of interest. This protocol describes the basic steps for lysing cells, determining total protein concentration in the lysate, running a precast SDS-PAGE gel, and immunoblotting. Sharing speeds science.
