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Recombination is the production of new DNA molecule(s) from two parental DNA molecules or different segments of the same DNA molecule; this will be the topic of this chapter.
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- Historical Development
- Genes That Encode Recombination Functions
- Recombination Enzymes
A simple example of recombination is diagramed in Fig. 1. A diploid organism has two homologous copies of a single chromosome that are identical except at two genetic loci, A and B. One chromosome has the alleles indicated by A and B, while the other has alleles a and b. In the absence of recombination, all progeny chromosomes that are A are also B...
The development of recombination mechanisms in the 1970s and 1980s benefitted from identification and characterization of the enzymes involved in homologous recombination during that time. Each mechanism requires the action of multiple types of enzymatic activities, some of which were hypothetical when the model was formulated. Genes encoding recom...
RecA and Other Recombinases
The bacterial RecA protein was purified by several labs in the 1970s and it has been the subject of intensive study ever since. Two eukaryotic RecA homologues, the Rad51 and DMC1 proteins, were recognized in 1992 (Bishop et al. 1992; Shinohara et al. 1992), and homologues are found in archaea (RadA) and in bacteriophage T4 (UvsX) (Liu et al. 2011). The RecA homologues alone catalyze the DNA strand exchange reaction that is the fundamental step in all recombination mechanisms going back to Hol...
RecBCD Enzyme
RecBCD enzyme acts in the eponymous RecBCD pathway for homologous recombination in bacteria, originally characterized as the major pathway for recombination during conjugation. RecBCD is now known to act in the recombinational repair of double-stranded DNA breaks that can arise during DNA replication or as a consequence of ionizing radiation. The RecBCD enzyme was first detected as a nuclease in E. coli and other bacteria that was dependent on ATP and magnesium ion for its activity (Clark 197...
Mechanism of Double-Strand Break Repair in Bacteria
RecA protein alone is able to carry out the key steps of homologous recombination, namely, strand invasion and branch migration to form and extend regions of heteroduplex DNA. Nonetheless, RecA and related recombinases are assisted by many other proteins in the complete process of homologous recombination. RecA must be provided with single-stranded DNA to form the RecA-ssDNA filament. In addition, cells contain other proteins that bind to ssDNA, namely, SSB (single-stranded DNA-binding protei...
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Recombination is the major and most basic factor that increases and decreases chromosomal and genetic components. I would like to review studies on homologous and non-homologous recombination, meiotic and somatic recombination, and the applications of basic mechanisms of recombination today, such as genetically modified plants and foods.
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DNA recombination and the movement of special DNA sequences in our chro-mosomes called transposable elements. THE MAINTENANCE OF DNA SEQUENCES Although the long-term survival of a species is enhanced by occasional genetic changes, the survival of the individual demands genetic stability. Only rarely do
Mar 3, 2022 · The book introduces the central players in recombination. An overview of the four major pathways of homologous recombinational repair is followed by a description of the several mechanisms of nonhomologous end-joining.
Jun 20, 2023 · General recombination is an integral part of the complex process of meiosis in sexually reproducing organisms. It results in a crossing over between pairs of genes along a chromosome, which are revealed in appropriate matings (Chapter 1).
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Feb 10, 2007 · Buffett, Peter - Recombination - Amazon.com Music ... Books, art & collectibles: ACX ... Indie Digital & Print Publishing Made Easy
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