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Jan 6, 2022 · These studies were key to the development of genetically modified potatoes with reduced acrylamide. The first generation Innate® potatoes were developed from cv. Russet Burbank by reducing Asn1 expression through RNAi, using tuber-specific expression promoters.
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Scheme of the process of silencing a gene by interference....
- Table 2
Cry3A gene introduction: 1995 (US, CAN) NewLeaf™ Plus:...
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Potato (Solanum tuberosum L.) is the third most important...
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As pollen and seeds move in the environment, they can...
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Jan 10, 2022 · Genetically engineered (GE) potato plants, obtained by classical genetic transformation strategies or genome editing tools, with increased resistance to insects, bacteria, fungi, viruses, herbicides, abiotic factors, and/or improved nutritional and post-harvest quality were developed.
- 10.3389/fpls.2021.768233
- 2021
- Front Plant Sci. 2021; 12: 768233.
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Jan 2, 2021 · These problems can be addressed by genetic modification (GM) or gene editing (GE) and open a wide horizon for potato crop improvement. Current genetically modified and gene edited varieties include those with Colorado potato beetle and late blight resistance, reduction in acrylamide, and modified starch content.
- Vigs
- Stable Rna-Mediated interference -Rnai
- Sigs
- Mutagenesis and Tilling
- Genome Editing
- Activation Tagging
VIGS is a rapid and high throughput technique for functional analysis of genes through transient post-transcriptional gene silencing (PTGS). In this method, a short cDNA sequence from the gene of interest is cloned into a VIGS vector, which is further transfected into the host plant via Agrobacterium tumefaciens or virus sap inoculation or RNA tran...
RNAi is a natural cellular process that downregulates the gene expression of the target gene by promoting the degradation of mRNA. It is a PTGS process and refers to a multi-step process, including the introduction of a DNA construct into a cell that produces dsRNA complementary to the gene of interest. This dsRNA is cleaved into ~ 21 to 25 nucleot...
SIGS is a powerful, fast, and environmentally friendly method for efficient crop protection and validation of gene function in plants. In this technique, RNAi based in vitro synthesized dsRNA molecules, targeting the virulence-related genes of pathogens molecules are topically applied onto the plants to silence the targeted genes of pathogens and i...
In mutagenesis, heritable changes are induced in the genetic information of an organism by chemical, physical and biological agents. Mainly, induced mutagenesis and insertion mutagenesis are employed in different mutation breeding programs. Induced mutagenesis is mostly instigated by radiations (gamma rays, X-rays, fast neutrons, etc.) or chemical ...
Sequence editing either by deleting or modifying the genes individually and then studying the subsequent mutant phenotypes could address the challenges of understanding the function of genes. For precise DNA manipulations, new genome editing systems have emerged in recent times. These can induce double-stranded breaks (DSBs) at specific sites in th...
Activation tagging is a powerful method to determine the function of a gene product by generating a gain of function mutants in plants that limits traditional screens for loss of function mutations. In this technique, T-DNA containing 4 tandem copies of multimerized transcriptional enhancers from the CaMV 35S gene is randomly integrated into the pl...
The recent approval of the Amflora potato by the European Union (EU)—the EU's first registration of a genetically modified (GM) potato in 12 years—has garnered considerable media attention...
Nov 4, 2019 · Now, researchers have for the first time shown they can reliably increase corn yields up to 10% by changing a gene that increases plant growth—regardless of whether growing conditions are poor or optimal.
