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- The CRISPR/Cas system has been applied to improve the genetic breeding and agricultural characteristics of potatoes and shown great potential in accelerating breeding, increasing yield, optimizing quality, and improving stress resistance.
www.ncbi.nlm.nih.gov › pmc › articlesCRISPR/Cas genome editing system and its application in potato
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Jan 6, 2022 · RNAi hairpin technology can be used to silence the haplo-alleles of multiple genes simultaneously, whereas optimization of newer gene editing technologies such as base and prime editing will facilitate the routine generation of advanced edits across the genome.
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Potato (Solanum tuberosum L.) is the third most important...
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Colorado potato beetle resistance: Cry3A gene introduction:...
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The editing system is attached to complementary target DNA...
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Feb 13, 2023 · In potato trait improvement, base editing technology can not only generate functionally acquired variants by changing individual bases but also achieve targeted evolution of specific genes by constructing sgRNA libraries to generate a library of mutations for a single gene.
- Xin Hou, Xiaomeng Guo, Yan Zhang, Qiang Zhang
- Front Genet. 2023; 14: 1017388.
- 10.3389/fgene.2023.1017388
- 2023
Mar 1, 2022 · Although transient application of genome editing has benefits such as low risk of obtaining chimeras and reduced risk of induced off-targets as well as yielding transgene-free events, stable transformation is today the predominant delivery method in research studies, plausibly due to the cumbersome process of isolation and regeneration from prot...
- Per Hofvander, Erik Andreasson, Mariette Andersson
- 2021
Apr 24, 2024 · Nature Reviews Genetics - Targeted genome modification using CRISPR–Cas genome editing, base editing or prime editing is driving base research in plants and precise molecular breeding. The...
Traits edited using CRISPR-Cas9 in potato. Genome editing can be used to control traits which can facilitate the manipulation of cultivars during breeding, improve the growth and the resilience of the cultivars in response to environmental cues, and finally manipulate the qualitative properties of the tuber based on the desired commercial ...
Dec 16, 2022 · Several genetic modifications including targeted mutations in a single gene or multiple genes, precise base editing and prime editing, gene knock-in and replacement, modulation of gene expression and epigenome editing can be achieved by CRISPR/Cas system.
With respect to the diverse traits of potato, there are many research reports using genome-editing technology. Starch composition, disease resistance, and long-term storage without sprout germination are important traits in potato.
