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The discovery and development of high-fidelity polymerases has for many years been a key focus at New England Biolabs (NEB). Highfidelity amplification is essential for experiments whose outcome depends upon the correct DNA sequence (e.g., cloning, SNP analysis, NGS applications).
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Mar 21, 2024 · The meaning of HIGH FIDELITY is the reproduction of an effect (such as sound or an image) that is very faithful to the original.
Nov 1, 2016 · Here we will focus on the structural and mechanistic features of DNA polymerases that allow them the synthesize DNA with high fidelity. Mammalian DNA polymerase beta (pol β), which is involved in base excision repair, is the smallest eukaryotic polymerase.
- M. Todd Washington
- 10.1016/j.str.2016.10.003
- 2016
- 2016/11/11
Feb 20, 2009 · These studies highlight a common problem in thinking about fidelity in the ribosome: what is the relationship between frameshifting, missense, and nonsense suppression? Should losses in fidelity necessarily affect all three phenomena, or is each process dictated by distinct features of the ribosome?
- Hani S. Zaher, Rachel Green
- 2009
The fidelity of a DNA polymerase refers to its ability to accurately replicate a template. A critical aspect of this is the ability of the DNA polymerase to read a template strand, select the appropriate nucleoside triphosphate and insert the correct nucleotide at the 3′ primer terminus, such that canonical Watson-Crick base pairing is ...
Feb 2, 2009 · According to their studies, occupancy of the E site with a cognate tRNA species results in a low affinity A site that discriminates effectively against non-cognate tRNA species, whereas an empty E site results in a high affinity and low fidelity A site.
Jul 26, 2005 · We have discussed two general mechanisms used to maintain the high fidelity of protein synthesis (as well as of DNA replication and transcription). The first mechanism is comprised of editing and kinetic proofreading.