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- The Van Slyke determination is a chemical test for the determination of amino acids containing a primary amine group. It is named after the biochemist Donald Dexter Van Slyke (1883-1971). One of Van Slyke's first professional achievements was the quantification of amino acids by the Van Slyke determination reaction.
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The Van Slyke determination is a chemical test for the determination of amino acids containing a primary amine group. It is named after the biochemist Donald Dexter Van Slyke (1883-1971). One of Van Slyke's first professional achievements was the quantification of amino acids by the Van Slyke determination reaction.
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Sep 15, 2010 · This method can be used to determine the amount of amino acids quantitatively, and the result is consistent with the determination using 2,4-dinitrofluorobenzene (DNFB, the Sanger method) and ninhydrin. However, there are certain amino acids in the protein, which cannot be determined.
Sep 6, 2015 · With this, the Van Slyke method tends to produce slight errors as the pCO 2 changes. That said, the SBE, unlike actual base excess (ABE), is extremely stable over wide ranges of PCO2. Additionally, the Van Slyke equation does not factor in the concentrations of phosphate and albumin, which play a role in the acid-base balance.
To this end, he devised a simple gasometric method for measuring accurately the concentration of sodium bicarbonate in a milliliter of blood. For this assay he invented the Van Slyke volumetric gas apparatus, which was soon found to be so useful that it was found in virtually all clinical laboratories and many biochemistry laboratories as well.
- Robert D. Simoni, Robert L. Hill, Martha Vaughan
- 2002
One method of analyzing amino acids is the van Slyke method. The characteristic amino groups (−NH2) in protein material are allowed to react with nitrous acid, HNO2, to form N2 gas. From the...
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Jul 8, 2024 · The meaning of VAN SLYKE METHOD is any of several analytical methods; especially : the determination of free amino groups (as in amino acids or proteins) by measuring the volume or pressure of nitrogen gas formed by reaction with nitrous acid.
…a procedure known as the Van Slyke method. With aromatic primary amines, nitrogen is not lost if the reaction mixture is kept cool (usually 0 °C [32 °F]), and a diazonium salt, ArN 2 + X − , where Ar is an aryl group, is formed:
