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1. • • The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.
     europepmc.org › article › MED

     The Solanum tuberosum GBSSI gene: a target for assessing gene ...

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   What is the Solanum tuberosum GBSSI gene?

   • The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.

   The Solanum tuberosum GBSSI gene: a target for assessing

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   Can the stgbssi gene be used in elite potato cultivars?

   • Our present work confirms that the StGBSSI gene can be successfully targeted and altered in elite potato cultivars using gene- and base-editing systems. Therefore, the StGBSSI appears to be a very good—and economically feasible—gene model to assess genome editing in potato, as well as its potential future technological improvements.

   The Solanum tuberosum GBSSI gene: a target for assessing ... - Springer

   link.springer.com/article/10.1007/s00299-019-02426-w

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   Can a stgbssi gene be targeted in a starch potato?

   • As a direct application of our work on Desiree, a cultivar commonly used in laboratories for many years, we targeted the StGBSSI gene through protoplast transfection in the Furia cultivar, a recently registered starch potato.

   The Solanum tuberosum GBSSI gene: a target for assessing ... - Springer

   link.springer.com/article/10.1007/s00299-019-02426-w

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   Can tetraploid potatoes be genetically edited?

   • The StGBSSI gene was successfully and precisely edited in the tetraploid potato using gene and base-editing strategies, leading to plants with impaired amylose biosynthesis. Genome editing has recently become a method of choice for basic research and functional genomics, and holds great potential for molecular plant-breeding applications.

   The Solanum tuberosum GBSSI gene: a target for assessing ... - Springer

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3. pubmed.ncbi.nlm.nih.gov › 31101972The Solanum tuberosum GBSSI gene: a target for assessing gene and...

   pubmed.ncbi.nlm.nih.gov › 31101972

   The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato. Plant Cell Rep. 2019 Sep;38 (9):1065-1080. doi: 10.1007/s00299-019-02426-w. Epub 2019 May 17.

   • Author: Florian Veillet, Laura Chauvin, Marie-Paule Kermarrec, François Sevestre, Mathilde Merrer, Zoé Terre...

   • Publish Year: 2019

4. link.springer.com › article › 10The Solanum tuberosum GBSSI gene: a target for assessing gene ...

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   May 17, 2019 · In this work, we targeted the StGBSSI gene to assay various CRISPR-Cas9 tools using stable and transient expression in different varieties of the tetraploid potato Solanum tuberosum. We identified single- and multi-allelic edited plants, and mutations in all four alleles were observed, resulting in amylose biosynthesis impairment.

   • Author: Florian Veillet, Laura Chauvin, Marie-Paule Kermarrec, François Sevestre, Mathilde Merrer, Zoé Terre...

   • Publish Year: 2019

5. link.springer.com › content › pdfThe Solanum tuberosum GBSSI gene: a target for assessing gene ...

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   Using ICE software, the mutants can be theoretically classified in four categories in a tetraploid species such as potato: ICE 25% (one allele likely to be mutated), ICE 50% (two alleles likely mutated), ICE 75% (three alleles likely mutated) and ICE 100% (four alleles likely mutated).

   • Author: Florian Veillet, Laura Chauvin, Marie-Paule Kermarrec, François Sevestre, Mathilde Merrer, Zoé Terre...

   • Publish Year: 2019

6. www.biorxiv.org › content › 10The Solanum tuberosum GBSSI gene: a target for assessing gene ...

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   May 5, 2019 · In this study, we produced mutants in two cultivated elite cultivars of the tetraploid potato ( Solanum tuberosum ) using stable or transient expression of the CRISPR-Cas9 components to knockout the amylose-producing StGBSSI gene.

   • Author: Florian Veillet, Laura Chauvin, Marie-Paule Kermarrec, François Sevestre, Mathilde Merrer, Zoé Terre...

   • Publish Year: 2019

7. www.researchgate.net › publication › 332892394_The(PDF) The Solanum tuberosum GBSSI gene: a target for ...

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   May 5, 2019 · The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato. May 2019. DOI: 10.1101/628107. License. CC BY-NC-ND 4.0. Authors: Florian...

8. www.semanticscholar.org › paper › The-Solanum[PDF] The Solanum tuberosum GBSSI gene: a target for ...

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   May 5, 2019 · The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato. F. Veillet, L. Chauvin, +7 authors. J. Chauvin. Published in Plant Cell Reports 5 May 2019. Biology, Environmental Science, Agricultural and Food Sciences. TLDR.

9. europepmc.org › article › MEDThe Solanum tuberosum GBSSI gene: a target for assessing gene ...

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   May 17, 2019 · The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato. Veillet F1, Chauvin L1, Kermarrec MP1, Sevestre F2, Merrer M1, Terret Z3, Szydlowski N2, Devaux P4, Gallois JL3, Chauvin JE1. Author information. Affiliations. 5 authors. 1.