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5 days ago · Western Blot Analysis is the most widely used technique for protein detection. In this video, we will cover the 3 major steps in generating a western blot: Separate, Transfer, and Detect.
- 3 min
5 days ago · The western blot is a research linchpin across all biomedical fields. While the overall workflow is the same, labs are swapping homemade reagents for commercial, clunky running systems for streamlined, and film for digital.
2 days ago · Western blot analysis Purified protein or cell lysates were subjected to SDS-PAGE and transferred onto a nitrocellulose membrane. The membrane was blocked using 1× PBS with 0.1% v/v Tween-20 and 5% w/v nonfat milk and then probed with primary antibodies specific to the epitope tags incorporated in the pJoseph2 plasmids ( Table 2 ).
3 days ago · Western blotting is a stalwart technique for analyzing specific proteins and/or their post-translational modifications. However, it remains challenging to accommodate more than ~10 samples per experiment without substantial departure from trusted, established protocols involving accessible instrumentation. Here, we describe a 96-sample western blot that conforms to standard 96-well plate ...
1 day ago · The supernatants were collected after 1 h and detected via Western blot assay using an anti-SARS-CoV-2 S1 polyclonal antibody. Experiments were performed twice, and one representative was displayed.
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5 days ago · A thick paper recommended for the lysis/denaturation of colony or plaque lifts and Western blots. Features and benefits: Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind. Ensures that no contamination will occur during the transfer steps.
2 days ago · E, F Western blot and densitometric analysis of TRIM33 protein expression in the NC (n = 5), EU (n = 7), and EC (n = 12) tissues; Data are presented as the mean ± SD. G , H Western blot and densitometric analysis of TRIM33, TGFBR1, SMAD2, p-SMAD2, α-SMA, FAP, FN1, and Collagen1 protein expression in the siNC and siTRIM33 transfected hESCs.
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