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Gram stain ( Gram staining or Gram's method ), is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. It may also be used to diagnose a fungal infection. [1] . The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique in 1884. [2]
E. coli stains gram-negative because its cell wall is composed of a thin peptidoglycan layer and an outer membrane. During the staining process, E. coli picks up the color of the counterstain safranin and stains pink.
Apr 13, 2016 · This paper reviewed core concepts of interpreting bacterial culture results, including timing of cultures, common culture sites, potential for contamination, interpreting the Gram stain, role of rapid diagnostic tests, conventional antibiotic susceptibility testing, and automated testing. Conclusion.
- Christopher Giuliano, Chandni R Patel, Pramodini B Kale-Pradhan
- CrossRef
- 2019
- P T. 2019 Apr; 44(4): 192-200.
Overview. What is a Gram stain? A Gram stain is a laboratory test that checks for bacteria at the site of a suspected infection or in certain bodily fluids. A medical laboratory scientist processes the Gram stain, which gives relatively quick results, so healthcare providers can know if bacteria are present, and, if so, the general type (s).
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Aug 14, 2023 · Introduction. The Gram staining is one of the most crucial staining techniques in microbiology. It gets its name from the Danish bacteriologist Hans Christian Gram, who first introduced it in 1882, mainly to identify organisms causing pneumonia. [1] .
- Nishant Tripathi, Amit Sapra
- 2023/08/14
Aug 10, 2022 · Interpretation. Gram Positive: Blue/Purple Color. Gram Negative: Red Color. Examples. Gram Positive Bacteria: Actinomyces, Bacillus, Clostridium, Corynebacterium, Enterococcus, Gardnerella, Lactobacillus, Listeria, Mycoplasma, Nocardia, Staphylococcus, Streptococcus, Streptomyces ,etc.
Dec 31, 2022 · Escherichia coli have served as important model organisms for over a century—used to elucidate key aspects of genetics, evolution, molecular biology, and pathogenesis. However, defining which strains actually belong to this species is erratic and unstable due to shifts in the characters and criteria used to distinguish bacterial species.
