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  2. E. coli is the most widely studied prokaryotic model organism, and an important species in the fields of biotechnology and microbiology, where it has served as the host organism for the majority of work with recombinant DNA. Under favourable conditions, it takes as little as 20 minutes to reproduce.

    • Overview
    • Key points:
    • Introduction
    • A prokaryote "family tree"
    • Bacteria
    • Archaea
    • The many "mystery prokaryotes"
    • What is a prokaryotic species?
    • Metagenomics: A new window on microbes

    Different groups of prokaryotes. Evolutionary relationships of bacteria and archaea. Extremophiles.

    •The two prokaryote domains, Bacteria and Archaea, split from each other early in the evolution of life.

    •Bacteria are very diverse, ranging from disease-causing pathogens to beneficial photosynthesizers and symbionts.

    •Archaea are also diverse, but none are pathogenic and many live in extreme environments.

    •The two prokaryote domains, Bacteria and Archaea, split from each other early in the evolution of life.

    •Bacteria are very diverse, ranging from disease-causing pathogens to beneficial photosynthesizers and symbionts.

    •Archaea are also diverse, but none are pathogenic and many live in extreme environments.

    •A DNA sequencing approach called metagenomics lets scientists identify new species of bacteria and archaea, including ones that can't be cultured.

    Prokaryotes, which include both bacteria and archaea, are found almost everywhere – in every ecosystem, on every surface of our homes, and inside of our bodies! Some live in environments too extreme for other organisms, such as hot vents on the ocean floor.

    Although they are found all around us, prokaryotes can be hard to detect, count, and classify. The prokaryotic species we know of today are a tiny fraction of all prokaryotic species thought to exist.1‍  In fact, the very idea of a “species” becomes complicated in the world of prokaryotes!

    For a long time, all prokaryotes were classified into a single domain (the largest taxonomic grouping).

    However, work by microbiologist Carl Woese in the 1970s showed that prokaryotes are divided into two distinct lineages, or lines of descent: Archaea and Bacteria. Today, these groups are considered to form two out of three domains of life. The third domain (Eukarya) includes all eukaryotes, such as plants, animals, and fungi.2‍ 

    [How did Woese figure this out?]

    Since splitting off from one another millions of years ago, both Bacteria and Archaea have split off into many groups and species.

    Domain Bacteria contains 5‍  major groups: proteobacteria, chlamydias, spirochetes, cyanobacteria, and gram-positive bacteria.

    The proteobacteria are subdivided into five groups, alpha through epsilon. Species in these groups have a wide range of lifestyles. Some are symbiotic with plants, others live in hot vents deep under the sea, and others yet cause human diseases, such as stomach ulcers (Helicobacter pylori) and food poisoning (Salmonella).

    Domain Archaea contains 4‍  major groups. Intriguingly, so far, no archaea that are human pathogens have yet been discovered.

    Archaea do live in our bodies and those of animals—for instance, in the gut—but all of them seem to be harmless or beneficial. Although there are hypotheses, no one yet knows exactly why archaea are all "friendly," i.e., why no disease-causing species have evolved.5‍ 

    Alongside the archaea that enjoy the comfy environment of the human gut, there are many extremophile species that live in much more inhospitable places. These include volcanic hot springs, undersea hot vents, and very salty places like the Dead Sea.

    [More about extremophiles]

    For many years, the main approach to studying prokaryotes was to grow them in the lab. If an organism could be grown on an agar plate or in a liquid culture, then it could be studied, analyzed, and added to our growing catalog of prokaryotic species and strains.

    Some prokaryotes, however, can't grow in a laboratory setting (at least, not under the conditions scientists have tried). In fact, an estimated 99%‍  of bacteria and archaea are unculturable!

    In order to talk about finding prokaryotic species, we probably need to define what they are. This may seem like a basic question, but it's a complex and even controversial one if you're a microbiologist.

    For eukaryotes, most scientists define a species as a group of organisms that can interbreed and have fertile offspring. This definition makes sense for species that reproduce sexually, but it doesn't work so well for organisms like bacteria. Bacteria reproduce asexually to make clones of themselves—they don't interbreed.

    Scientists estimate there may be millions of prokaryotic species (or species-like groups), but we know very little about most of them.1‍  This is starting to change thanks to large-scale DNA sequencing.

    DNA sequencing makes it possible for scientists to study entire prokaryotic communities in their natural habitats – including the many prokaryotes that are unculturable, and would previously have been "invisible" to researchers.

    The collective genome of such a community is called its metagenome, and the analysis of metagenome sequences is known as metagenomics. Prokaryotic metagenomics is one of the areas of biology that I find coolest and most mysterious.

    For example, a DNA sample can be taken from a hot spring microbial mat, such as the beautiful, multicolored mats found in Yellowstone National Park. Even a tiny sample from this rich community includes many, many individuals of different species.9‍ 

    By sequencing and analyzing metagenome DNA samples, scientists can sometimes piece together entire genomes of previously unknown species. In other cases, they use sequence information from specific genes to figure out what types of prokaryotes are present (and how they are related to each other or to known species). The genes found in the DNA samples can also provide clues about the metabolic strategies of the organisms in the community.10‍ 

    [Attribution and references]

  3. Key terms. How do prokaryotic and eukaryotic cells differ? What is the endosymbiotic theory? One theory that may explain how eukaryotes became so complex is the endosymbiotic theory. This theory proposes that organelles like mitochondria and chloroplasts were once free-living prokaryotic cells that began to live within a larger host cell.

  4. Key points: Prokaryotes (bacteria and archaea) reproduce asexually through binary fission. Most prokaryotes reproduce rapidly. Due to their fast growth and simple genetics, E. coli bacteria are widely used in molecular biology. In the laboratory, a gene can be transferred into E. coli bacteria on a small, circular DNA molecule called a plasmid.

  5. Nov 3, 2023 · According to scientists, the world is split into two kinds of organisms — prokaryotic vs. eukaryotic — which have two different types of cells. An organism can be made up of either one type or the other. Some organisms consist of only one measly cell, but even so, that cell will either be either prokaryotic or eukaryotic.

    • Jesslyn Shields
  6. The DNA of E. coli is a single circular molecule in the nucleoid, which, in contrast to the nucleus of eukaryotes, is not surrounded by a membrane separating it from the cytoplasm.

  7. Dec 31, 2022 · Those sequences with rare or sporadic distributions, as might originate from infrequent or independent events of horizontal gene transfer between taxa, occur in eukaryotes as well as bacteria ( Akanni et al. 2015; Husnik and McCutcheon 2018; Wu et al. 2022 ), and can involve very distant taxa.

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